Zhgun Alexander A, Potapov Mark P, Avdanina Darya A, Karpova Natalya V, Yaderets Vera V, Dzhavakhiya Vakhtang V, Kardonsky Dmitry A
Research Center of Biotechnology, Russian Academy of Sciences, 119071 Moscow, Russia.
Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, Russia.
Biology (Basel). 2022 Jun 8;11(6):883. doi: 10.3390/biology11060883.
The transformation of steroids by microorganisms is widely used in medical biotechnology. A huge group of filamentous fungi is one of the most promising taxa for screening new biocatalytic reactions in order to obtain pharmaceutically significant steroids. In this work, we screened 10 filamentous fungi-destructors of egg tempera for the ability to biotransform androst-4-en-3,17-dione (AD) during cultivation in a liquid nutrient medium or in a buffer solution. These taxonomically unrelated strains, belonging to the classes , and , are dominant representatives of the microbiome from halls where works of tempera painting are stored in the State Tretyakov Gallery (STG, Moscow, Russia). Since the binder of tempera paints, egg yolk, contains about 2% cholesterol, these degrading fungi appear to be a promising group for screening for steroid converting activity. It turned out that all the studied fungi-destructors are able to transform AD. Some strains showed transformation efficiency close to the industrial strain RNCIM F-981. In total, 33 steroids formed during the transformation of AD were characterized, for 19 of them the structure was established by gas chromatography/mass spectrometry analysis. In this work, we have shown for the first time that fungi-destructors of tempera paintings can efficiently transform steroids.
微生物对甾体的转化在医学生物技术中有着广泛应用。一大类丝状真菌是筛选新的生物催化反应以获得具有药学意义的甾体的最有前景的分类群之一。在这项工作中，我们筛选了10种蛋彩画破坏丝状真菌，以研究它们在液体营养培养基或缓冲溶液中培养时对雄甾-4-烯-3,17-二酮（AD）进行生物转化的能力。这些分类学上不相关的菌株，分别属于 、 和 纲，是俄罗斯莫斯科国立特列季亚科夫画廊（STG）中存放蛋彩画作品展厅微生物群落的主要代表。由于蛋彩画的粘合剂蛋黄中含有约2%的胆固醇，这些降解真菌似乎是筛选甾体转化活性的一个有前景的群体。结果表明，所有研究的破坏真菌都能够转化AD。一些菌株的转化效率接近工业菌株RNCIM F-981。在AD转化过程中总共鉴定出33种甾体，其中19种的结构通过气相色谱/质谱分析得以确定。在这项工作中，我们首次表明蛋彩画破坏真菌能够有效地转化甾体。
