Dauda Wadzani Palnam, Abraham Peter, Glen Elkanah, Adetunji Charles Oluwaseun, Ghazanfar Shakira, Ali Shafaqat, Al-Zahrani Majid, Azameti Mawuli Kwamla, Alao Sheik Emmanuel Laykay, Zarafi Afiniki Bawa, Abraham Maryam Peter, Musa Hannatu
Crop Science Unit, Department of Agronomy, Federal University Gashua, Gashua P.M.B 1005, Yobe State, Nigeria.
Department of Horticulture, Federal College of Horticulture, Dadin Kowa P.M.B 108, Gombe State, Nigeria.
Life (Basel). 2022 Mar 18;12(3):451. doi: 10.3390/life12030451.
Cytochrome P450s (P450ome) constitute an extended superfamily group of heme-thiolate enzymes identified in all biological domains. P450omes play a critical role in the oxidation of steroids and fatty acids, xenobiotic degradation of hydrophobic compounds, biosynthesis of hormones, and primary and secondary metabolism in organisms. species are among the most economically important fungal organisms in human medicine, industry, and agriculture worldwide. Exploring insight on the genome-wide annotations of cytochrome P450s in species is necessary for their biosynthetic applications. In this present study, we report the identification of 306 cytochrome P450s and their robust profiling in eight notable species (, , , , , , and ). Based on the evolutionary relationship, the P450s families clustered into 15 clades, with clades V, I, and XIII recording higher percentages (17.3%, 15.00%, and 14.71%, respectively) of Cyp families. Cyps were classified into 120 families 64 clans, and their putative functions were also elucidated. P450s were predicted to be located in 13 subcellular components, but the endoplasm reticulum was the dominant location across the eight species. Cyps genes of species were associated with seven secondary metabolism-related gene clusters. Elucidating the genome-wide annotations of P450s enzymes in species will form vital potential biotechnological tools that could be harnessed for industrial, pharmaceutical, and agricultural use.
细胞色素P450（P450ome）构成了在所有生物领域中都能鉴定出的血红素硫醇盐酶的一个扩展超家族。P450ome在类固醇和脂肪酸的氧化、疏水性化合物的异源生物降解、激素的生物合成以及生物体的初级和次级代谢中发挥着关键作用。在全球人类医学、工业和农业中，[物种名称]是最重要的经济真菌生物之一。探索[物种名称]中细胞色素P450的全基因组注释对于其生物合成应用是必要的。在本研究中，我们报告了在八个著名的[物种名称]（[具体物种1]、[具体物种2]、[具体物种3]、[具体物种4]、[具体物种5]、[具体物种6]、[具体物种7]和[具体物种8]）中鉴定出306种细胞色素P450及其详细分析。基于进化关系，[物种名称]的P450家族聚为15个分支，其中分支V、I和XIII的Cyp家族百分比更高（分别为17.3%、15.00%和14.71%）。Cyp被分为120个家族64个族，其推定功能也得到了阐明。P450预计位于13个亚细胞组分中，但内质网是这八个[物种名称]中的主要位置。[物种名称]的Cyp基因与七个次级代谢相关基因簇相关。阐明[物种名称]中P450酶的全基因组注释将形成重要的潜在生物技术工具，可用于工业、制药和农业用途。
