Khonchaisri Rattanaporn, Sumonsiri Nutsuda, Prommajak Trakul, Rachtanapun Pornchai, Leksawasdi Noppol, Techapun Charin, Taesuwan Siraphat, Halee Anek, Nunta Rojarej, Khemacheewakul Julaluk
Division of Food Science and Technology, School of Agro-Industry, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, Thailand.
Department of Agro-Industrial, Food and Environmental Technology, Faculty of Applied Science, King Mongkut's University of Technology North Bangkok, Bangkok 10800, Thailand.
Foods. 2022 Jun 16;11(12):1775. doi: 10.3390/foods11121775.
Green soybean ( L.) seeds (GSS) are rich in various antioxidants and phytonutrients that are linked to various health benefits. Ultrasound-assisted extraction (UAE) technology was used for extracting the effective components from GSS. A response surface method (RSM) was used to examine the influence of liquid-to-solid ratio and extraction temperature on the bioactive compounds and antioxidant characteristics. The optimal conditions were a liquid-to-solid ratio of 25:1 and a UAE temperature of 40 °C. The observed values coincided well with the predicted values under optimal conditions. Additionally, the effects of drying methods on the procyanidins and antioxidant activities of GSS extract were evaluated. The spray-dried GSS extract contained the highest levels of procyanidins (21.4 ± 0.37 mg PC/g), DPPH (199 ± 0.85 µM Trolox eq/g), and FRAP (243 ± 0.26 µM Trolox eq/g). Spray drying could be the most time- and energy-efficient technique for drying the GSS extract. The present study also assessed the effects of storage temperature and time on procyanidins and antioxidant activities in GSS extract powder. Procyanidins were found to degrade more rapidly at 45 °C than at 25 °C and 35 °C. Storage under 25 °C was appropriate for maintaining the procyanidin contents, DPPH, and FRAP activities in the GSS extract powder. This study contributed to the body of knowledge by explaining the preparation of procyanidin extract powder from GSS, which might be employed as a low-cost supply of nutraceutical compounds for the functional food industry and pharmaceutical sector.
青大豆（L.）种子（GSS）富含多种抗氧化剂和植物营养素，这些物质与多种健康益处相关。采用超声辅助提取（UAE）技术从GSS中提取有效成分。运用响应面法（RSM）研究液固比和提取温度对生物活性化合物及抗氧化特性的影响。最佳条件为液固比25:1，UAE温度40℃。在最佳条件下，观测值与预测值吻合良好。此外，还评估了干燥方法对GSS提取物中原花青素和抗氧化活性的影响。喷雾干燥的GSS提取物中原花青素含量最高（21.4±0.37 mg PC/g）、DPPH（199±0.85 µM Trolox当量/g）和FRAP（243±0.26 µM Trolox当量/g）。喷雾干燥可能是干燥GSS提取物最省时且节能的技术。本研究还评估了储存温度和时间对GSS提取物粉末中原花青素和抗氧化活性的影响。发现原花青素在45℃下比在25℃和35℃下降解更快。25℃储存适合保持GSS提取物粉末中的原花青素含量、DPPH和FRAP活性。本研究通过解释从GSS制备原花青素提取物粉末，为知识体系做出了贡献，该提取物粉末可作为功能食品工业和制药行业低成本的营养化合物供应来源。
