pgi基因的缺失改变了基因工程改造的大肠杆菌菌株中的色氨酸生物合成。
Deletion of pgi alters tryptophan biosynthesis in a genetically engineered strain of Escherichia coli.
作者信息
Mascarenhas D, Ashworth D J, Chen C S
机构信息
Western Research Center, Stauffer Chemical Company, Richmond, California 94804.
出版信息
Appl Environ Microbiol. 1991 Oct;57(10):2995-9. doi: 10.1128/aem.57.10.2995-2999.1991.
Abstract
Deletion of the structural gene for phosphoglucose isomerase (pgi) of Escherichia coli dramatically alters the path of glucose catabolism by diverting carbon into the hexose monophosphate shunt. The effect of this genetic alteration on the conversion of glucose to tryptophan by strains optimized for the biosynthesis of this amino acid was determined by using 13C-nuclear magnetic resonance spectroscopy in vivo. Pgi- strains converted glucose to tryptophan almost twice as efficiently as did their Pgi+ counterparts.
摘要
删除大肠杆菌磷酸葡萄糖异构酶（pgi）的结构基因，通过将碳分流到磷酸戊糖途径，极大地改变了葡萄糖的分解代谢途径。通过体内13C核磁共振光谱法，确定了这种基因改变对为该氨基酸生物合成而优化的菌株将葡萄糖转化为色氨酸的影响。Pgi-菌株将葡萄糖转化为色氨酸的效率几乎是其Pgi+对应菌株的两倍。
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