Departamento de Reproducción Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), CSIC, 28040 Madrid, Spain.
Centro de Investigación en Sanidad Animal (CISA), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), CSIC, 28130 Madrid, Spain.
Int J Mol Sci. 2022 Jun 8;23(12):6420. doi: 10.3390/ijms23126420.
Although the European rabbit is an "endangered" species and a notorious biological model, the analysis and comparative characterization of new tissue sources of rabbit mesenchymal stem cells (rMSCs) have not been well addressed. Here, we report for the first time the isolation and characterization of rMSCs derived from an animal belonging to a natural rabbit population within the native region of the species. New rMSC lines were isolated from different tissues: oral mucosa (rOM-MSC), dermal skin (rDS-MSC), subcutaneous adipose tissue (rSCA-MSC), ovarian adipose tissue (rOA-MSC), oviduct (rO-MSC), and mammary gland (rMG-MSC). The six rMSC lines showed plastic adhesion with fibroblast-like morphology and were all shown to be positive for CD44 and CD29 expression (characteristic markers of MSCs), and negative for CD34 or CD45 expression. In terms of pluripotency features, all rMSC lines expressed NANOG, OCT4, and SOX2. Furthermore, all rMSC lines cultured under osteogenic, chondrogenic, and adipogenic conditions showed differentiation capacity. In conclusion, this study describes the isolation and characterization of new rabbit cell lines from different tissue origins, with a clear mesenchymal pattern. We show that rMSC do not exhibit differences in terms of morphological features, expression of the cell surface, and intracellular markers of pluripotency and in vitro differentiation capacities, attributable to their tissue of origin.
虽然欧洲兔是一种“濒危”物种，也是一种臭名昭著的生物模型，但对新的兔间充质干细胞（rMSC）组织来源的分析和比较特征尚未得到很好的解决。在这里，我们首次报道了从属于物种本地范围内的天然兔群的动物中分离和鉴定 rMSC 的情况。从不同组织中分离出了新的 rMSC 系：口腔黏膜（rOM-MSC）、皮肤真皮（rDS-MSC）、皮下脂肪组织（rSCA-MSC）、卵巢脂肪组织（rOA-MSC）、输卵管（rO-MSC）和乳腺（rMG-MSC）。这 6 条 rMSC 系均表现出贴壁生长的成纤维样形态，均表达 CD44 和 CD29（MSC 的特征性标志物），不表达 CD34 或 CD45。就多能性特征而言，所有 rMSC 系均表达 NANOG、OCT4 和 SOX2。此外，所有 rMSC 系在成骨、成软骨和成脂条件下培养均表现出分化能力。总之，本研究描述了从不同组织来源分离和鉴定新型兔细胞系的情况，其具有明确的间充质特征。我们表明，rMSC 在形态特征、表面细胞表达和多能性的细胞内标志物以及体外分化能力方面没有差异，这归因于它们的组织来源。
