Petrov Lubomir, Alexandrova Albena, Argirova Mariana, Tomova Teodora, Georgieva Almira, Tsvetanova Elina, Mileva Milka
National Sports Academy "Vassil Levski", 21, Acad. Stefan Mladenov, Studentski Grad, 1700 Sofia, Bulgaria.
Department of Chemical Sciences, Faculty of Pharmacy, Medical University of Plovdiv, 4000 Plovdiv, Bulgaria.
Life (Basel). 2022 Jun 12;12(6):878. doi: 10.3390/life12060878.
Oxidative stress underlies the pathogenesis of many diseases, which determines the interest in natural substances with antioxidant properties. L. leaves are well known and widely used in the pharmaceutical industry, but the therapeutic properties of the seeds are less studied. This study aimed to identify the chromatographic profile and to evaluate the antioxidant properties of methanol extract from seeds of (GBSE). In the GBSE, flavonoids and terpenes were found as terpenes predominated. The GBSE antioxidant capacity determined by 2,2 azino-bis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) and 1-diphenyl-2-picrylhydrazyl (DPPH) methods were equal to 1.34% and 0.58% of the activity of reference substance Trolox, respectively. The results of the ferric reducing antioxidant power method showed that the effect of concentration 1 mg/mL (/) GBSE was equal to 7.418 mM FeSO used as a standard. The cupric reducing antioxidant capacity activity of the GBSE was found to be 215.39 µmol Trolox/g GBSE and is presented as Trolox equivalent. The metal chelation effect of 1 mg/mL (/) GBSE was equal to that obtained for 0.018 mM EDTA. In conclusion, GBSE showed a good ability to neutralize ABTS and DPPH radicals and could have a beneficial effect in pathological conditions with oxidative stress etiology.
氧化应激是许多疾病发病机制的基础，这决定了人们对具有抗氧化特性的天然物质的兴趣。L. 的叶子广为人知并在制药行业广泛应用，但种子的治疗特性研究较少。本研究旨在鉴定 (GBSE) 种子甲醇提取物的色谱图谱并评估其抗氧化特性。在GBSE中，发现了黄酮类化合物和萜类化合物，其中萜类化合物占主导。通过2,2 - 联氮 - 双 (3 - 乙基苯并噻唑啉 - 6 - 磺酸) (ABTS) 和1,1 - 二苯基 - 2 - 苦基肼 (DPPH) 方法测定的GBSE抗氧化能力分别相当于参考物质Trolox活性的1.34% 和0.58%。铁还原抗氧化能力方法的结果表明，浓度为1 mg/mL (/) 的GBSE的效果相当于用作标准的7.418 mM硫酸亚铁。GBSE的铜还原抗氧化能力活性为215.39 μmol Trolox/g GBSE，并以Trolox当量表示。1 mg/mL (/) 的GBSE的金属螯合效应与0.018 mM乙二胺四乙酸 (EDTA) 的效果相当。总之，GBSE表现出良好的中和ABTS和DPPH自由基的能力，并且在具有氧化应激病因的病理状况下可能具有有益作用。
