Alamery Salman, Zargar Seema, Yaseen Fatimah, Wani Tanveer A, Siyal Abdulaziz
Department of Biochemistry, College of Science, King Saud University, Riyadh 11495, Saudi Arabia.
Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia.
Life (Basel). 2022 Jun 15;12(6):900. doi: 10.3390/life12060900.
Thioacetamide (TAA) intoxication produces a reproducible standard animal model of induced liver and kidney injuries where free radicals are produced by phase I oxidation reactions, which eventually leads to liver and kidney failure. Wheat germ oil (WGO) is a unique food supplement with concentrated nutrient efficiency and has remarkable antioxidant functions. Olmutinib, on the other hand, is a chemotherapy drug considered safe for kidneys and the liver. Therefore, in this study, WGO and olmutinib were investigated for their effect on TAA-induced liver and kidney damage. Inflammatory markers; interleukin-1 beta (IL-1β); IL-6; and the levels of enzymatic markers ALT (Alanine aminotransferase), AST (Aspartate aminotransferase), LDH (Lactate dehydrogenase), and CK (creatinine kinase) in serum for liver and kidney were analyzed and evaluated along with histopathological changes in the tissue. Thirty male mice 4-6 weeks of age were grouped into five groups of six animals: the control group (saline) and the other groups (Groups II to V), which were given thioacetamide for two weeks. In addition, Group II continued with TAA; Group III was given olmutinib (30 mg/kg), Group IV was given the wheat germ oil (WGO) (1400 mg/kg), and Group V was given (olmutinib (30 mg/kg) + WGO (1400 mg/kg)) for five days. The results suggested that olmutinib treatment potentiated TAA-induced liver and kidney injury. At the same time, WGO efficiently alleviated TAA and TAA-olmutinib toxicity in Groups IV and V. The histological studies also showed reduced damage with WGO in the animal model. Hence, it was concluded that WGO could significantly reduce liver and kidney damage caused by TAA and olmutinib in mice.
硫代乙酰胺（TAA）中毒可产生一种可重复的诱导肝损伤和肾损伤的标准动物模型，其中自由基通过I相氧化反应产生，最终导致肝肾功能衰竭。小麦胚芽油（WGO）是一种具有浓缩营养功效的独特食品补充剂，具有显著的抗氧化功能。另一方面，奥莫替尼是一种被认为对肝肾安全的化疗药物。因此，在本研究中，对WGO和奥莫替尼对TAA诱导的肝损伤和肾损伤的影响进行了研究。分析并评估了炎症标志物白细胞介素-1β（IL-1β）、IL-6以及血清中肝肾功能酶标志物谷丙转氨酶（ALT）、谷草转氨酶（AST）、乳酸脱氢酶（LDH）和肌酐激酶（CK）的水平，以及组织中的组织病理学变化。将30只4 - 6周龄的雄性小鼠分为五组，每组6只：对照组（生理盐水）和其他组（第二组至第五组），后四组给予硫代乙酰胺两周。此外，第二组继续给予TAA；第三组给予奥莫替尼（30 mg/kg），第四组给予小麦胚芽油（WGO）（1400 mg/kg），第五组给予（奥莫替尼（30 mg/kg）+WGO（1400 mg/kg）），持续五天。结果表明，奥莫替尼治疗会增强TAA诱导的肝损伤和肾损伤。同时，WGO有效减轻了第四组和第五组中TAA和TAA - 奥莫替尼的毒性。组织学研究还显示动物模型中WGO减轻了损伤。因此，得出结论：WGO可显著降低TAA和奥莫替尼对小鼠造成的肝损伤和肾损伤。
