Institute of Microtechnology, Technische Universität Braunschweig, Braunschweig, Germany.
Center of Pharmaceutical Engineering (PVZ), Technische Universität Braunschweig, Braunschweig, Germany.
Electrophoresis. 2020 Jan;41(1-2):65-80. doi: 10.1002/elps.201900286. Epub 2019 Nov 8.
Electrical impedance is an established technique used for cell and particle characterization. The temporal and spectral resolution of electrical impedance have been used to resolve basic cell characteristics like size and type, as well as to determine cell viability and activity. Such electrical impedance measurements are typically performed across the entire sample volume and can only provide an overall indication concerning the properties and state of that sample. For the study of heterogeneous structures such as cell layers, biological tissue, or polydisperse particle mixtures, an overall measured impedance value can only provide limited information and can lead to data misinterpretation. For the investigation of localized sample properties in complex heterogeneous structures/mixtures, the addition of spatial resolution to impedance measurements is necessary. Several spatially resolved impedance measurement techniques have been developed and applied to cell and particle research, including electrical impedance tomography, scanning electrochemical microscopy, and microelectrode arrays. This review provides an overview of spatially resolved impedance measurement methods and assesses their applicability for cell and particle characterization.
阻抗是一种用于细胞和颗粒特征分析的成熟技术。阻抗的时间和频谱分辨率已被用于解析基本的细胞特性，如大小和类型，以及确定细胞活力和活性。这种阻抗测量通常在整个样品体积上进行，只能提供关于该样品的特性和状态的总体指示。对于细胞层、生物组织或多分散颗粒混合物等不均匀结构的研究，整体测量的阻抗值只能提供有限的信息，并可能导致数据解释错误。为了研究复杂不均匀结构/混合物中的局部样品特性，需要在阻抗测量中增加空间分辨率。已经开发并应用了几种空间分辨阻抗测量技术，包括阻抗断层成像、扫描电化学显微镜和微电极阵列。本文综述了空间分辨阻抗测量方法，并评估了它们在细胞和颗粒特征分析中的适用性。
