Shapiro David, Kapourchali Fatemeh Ramezani, Santilli Anthony, Han Yingchun, Cresci Gail A M
Department of Inflammation and Immunity, Cleveland Clinic, Cleveland, OH 44195, USA.
Department of Pediatric Gastroenterology, Cleveland Clinic, Cleveland, OH 44195, USA.
Microorganisms. 2022 Jun 8;10(6):1178. doi: 10.3390/microorganisms10061178.
Antibiotic therapy is necessary for the treatment of bacterial infections; however, it can also disrupt the balance and function of commensal gut microbes and negatively affect the host. Probiotics have been tested as a means to counteract the negative effects of antibiotic therapy, but many probiotics are also likely destroyed by antibiotics when taken together. Here we aimed to test the efficacy of a non-pathogenic spore-forming -species containing a probiotic blend provided during antibiotic therapy on host immune defenses in mice. Mice were exposed to antibiotics and supplemented with or without the probiotic blend and compared to control mice. Fecal and cecal contents were analyzed for gut microbes, and intestinal tissue was tested for the expression of key enzymes involved in vitamin A metabolism, serum amyloid A, and inflammatory markers in the intestine. The probiotic blend protected against antibiotic-induced overgrowth of gram-negative bacteria and gammaproteobacteria in the cecum which correlated with host immune responses. Regional responses in mRNA expression of enzymes involved with vitamin A metabolism occurred between antibiotic groups, and intestinal inflammatory markers were mitigated with the probiotic blend. These data suggest prophylactic supplementation with a spore-forming -containing probiotic may protect against antibiotic-induced dysregulation of host immune responses.
抗生素治疗对于细菌感染的治疗是必要的；然而，它也会破坏共生肠道微生物的平衡和功能，并对宿主产生负面影响。益生菌已被作为一种抵消抗生素治疗负面影响的手段进行测试，但许多益生菌与抗生素一起服用时也可能被抗生素破坏。在这里，我们旨在测试一种在抗生素治疗期间提供的含有益生菌混合物的非致病性芽孢形成菌对小鼠宿主免疫防御的功效。将小鼠暴露于抗生素中，并补充或不补充益生菌混合物，然后与对照小鼠进行比较。分析粪便和盲肠内容物中的肠道微生物，并检测肠道组织中参与维生素A代谢、血清淀粉样蛋白A和肠道炎症标志物的关键酶的表达。益生菌混合物可防止抗生素诱导的盲肠中革兰氏阴性菌和γ-变形菌过度生长，这与宿主免疫反应相关。抗生素组之间发生了与维生素A代谢相关酶的mRNA表达的区域反应，并且益生菌混合物减轻了肠道炎症标志物。这些数据表明，预防性补充含芽孢形成菌的益生菌可能预防抗生素诱导的宿主免疫反应失调。
