Gajera Gemini, Funde Snehal, Palep Hanmanthrao, Kothari Vijay
Institute of Science, Nirma University, Ahmedabad, 382481, India.
Dr. Palep's Medical Research Foundation, Mumbai, India.
J Ayurveda Integr Med. 2024 Mar-Apr;15(2):100880. doi: 10.1016/j.jaim.2023.100880. Epub 2024 Mar 8.
This study aimed at investigating whether the duration of fermenting Panchagavya (PG) preparation in copper vessel affects its biological activity and microbiome composition.
Prophylactic potential of PG against bacterial infection was assessed through an in vivo assay employing the nematode worm Caenorhabditis elegans as a model host. Bacterial diversity of the PG samples was revealed through metagenomic analysis.
Duration of fermentation was found to affect biological activity as well as microbiome composition of the PG samples. PG-samples fermented ≥60 min lost their prophylactic potential, and develop anthelmintic activity. Bacterial phyla whose relative abundance was significantly different between the prophylactic and anthelmintic PG samples were Planctomycetota, Proteabacteria, Bacteroidota, Verrucomicrobiota, Patescibacteria, Acidobacteriota, Chloroflexi, Firmicutes and Campilobacterota.
This study validates the prophylactic potential of Panchagavya against bacterial pathogens, and shows that duration of the fermentation time while preparing PG can have profound effect on its biological activities. Biological activities of PG samples seem to have a correlation with their inherent microbial community. Metagenomic profiling can be an effective tool for standardization of PG formulations.
本研究旨在调查在铜容器中发酵五灵脂（PG）制剂的时间是否会影响其生物活性和微生物群落组成。
通过以线虫秀丽隐杆线虫为模型宿主的体内试验评估PG对细菌感染的预防潜力。通过宏基因组分析揭示PG样品的细菌多样性。
发现发酵时间会影响PG样品的生物活性以及微生物群落组成。发酵≥60分钟的PG样品失去了其预防潜力，并产生了驱虫活性。在具有预防作用和驱虫作用的PG样品之间，相对丰度存在显著差异的细菌门有浮霉菌门、变形菌门、拟杆菌门、疣微菌门、Patescibacteria、酸杆菌门、绿弯菌门、厚壁菌门和弯曲杆菌门。
本研究验证了五灵脂对细菌病原体的预防潜力，并表明制备PG时的发酵时间长短会对其生物活性产生深远影响。PG样品的生物活性似乎与其固有的微生物群落相关。宏基因组分析可成为PG制剂标准化的有效工具。
