Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Veterinary Medicine, Shandong Agricultural University, Taian 271018, China.
College of Chemistry and Material Science, Shandong Agricultural University, Taian 271018, China.
Molecules. 2022 Jun 20;27(12):3942. doi: 10.3390/molecules27123942.
Biosensor analysis based on the surface plasmon resonance (SPR) phenomenon enables label-free, highly sensitive analyte detection without prior sample purification or processing. However, potential applications of SPR biosensors in virus detection in biological samples remain to be explored. Owing to its excellent biocompatibility and abundance of hydroxyl and carboxyl functional groups, graphene oxide (GO) has been widely used as a biosensor of proteins and metal ions in living cells. The present work explored the effect of GO modification on the sensitivity of an SPR biosensor and used a GO-modified sensor to detect porcine reproductive and respiratory syndrome virus in cell culture, as shown. The GO modification markedly enhanced the sensitivity of the Fourier transform SPR sensor and enabled linear detection of porcine reproductive and respiratory syndrome virus (PRRSV) with a multiplicity of infection in the range 0.2-1.7 ( = 0.998). Such a GO-modified sensor provides a promising alternative for virus detection.
基于表面等离子体共振 (SPR) 现象的生物传感器分析可实现无需样品纯化或预处理的无标记、高灵敏度分析物检测。然而，SPR 生物传感器在生物样品中病毒检测的潜在应用仍有待探索。由于其出色的生物相容性和丰富的羟基和羧基官能团，氧化石墨烯 (GO) 已被广泛用作活细胞中蛋白质和金属离子的生物传感器。本工作探讨了 GO 修饰对 SPR 生物传感器灵敏度的影响，并使用 GO 修饰的传感器检测细胞培养中的猪繁殖与呼吸综合征病毒，结果表明：GO 修饰显著提高了傅里叶变换 SPR 传感器的灵敏度，并能够线性检测感染复数在 0.2-1.7 范围内的猪繁殖与呼吸综合征病毒 (PRRSV)（=0.998）。这种 GO 修饰的传感器为病毒检测提供了一种有前途的替代方法。
