鲨肌肽与头孢他啶、左氧氟沙星、复方新诺明和黏菌素联合使用对机会致病菌的杀菌活性
Bactericidal Activity of Ceragenin in Combination with Ceftazidime, Levofloxacin, Co-Trimoxazole, and Colistin against the Opportunistic Pathogen .
作者信息
Paprocka Paulina, Mańkowska Angelika, Skłodowski Karol, Król Grzegorz, Wollny Tomasz, Lesiak Agata, Głuszek Katarzyna, Savage Paul B, Durnaś Bonita, Bucki Robert
机构信息
Department of Microbiology and Immunology, Institute of Medical Science, Collegium Medicum, Jan Kochanowski University, IX Wieków Kielc 19A, 25-317 Kielce, Poland.
Department of Medical Microbiology and Nanobiomedical Engineering, Medical University of Białystok, Jana Kilińśkiego 1 Białystok, 15-089 Białystok, Poland.
出版信息
Pathogens. 2022 May 26;11(6):621. doi: 10.3390/pathogens11060621.
BACKGROUND
() is an emerging opportunistic Gram-negative rod causing nosocomial infections predominantly in immunocompromised patients. Due to its broad intrinsic resistance to antibiotics, including carbapenems and the ability to form a biofilm, it is difficult to eradicate.
METHODS
In this study, the benefit of combined administration (potential synergism) and anti-biofilm activity of ceragenins: CSA-13, CSA-44, and CSA-131 (synthetic mimics of natural antimicrobial peptides) with ceftazidime, levofloxacin, co-trimoxazole and colistin against clinical strains of were determined using MIC/MBC (minimum inhibitory concentration/minimum bactericidal concentration), killing assays and CV staining.
RESULTS
Obtained data indicate that the ceragenins exhibit strong activity against the tested strains of grown in planktonic culture and as stationary biofilms. Moreover, with some strains, the synergy of ceragenins with conventional antibiotics was observed Conclusion: Our data suggest that ceragenins are promising agents for future development of new methods for treatment of infections caused by , along with its potential use in combination with conventional antibiotics.
背景
（某菌名未给出）是一种新兴的机会性革兰氏阴性杆菌，主要在免疫功能低下的患者中引起医院感染。由于其对包括碳青霉烯类在内的多种抗生素具有广泛的固有耐药性，且具有形成生物膜的能力，因此难以根除。
方法
在本研究中，使用MIC/MBC（最低抑菌浓度/最低杀菌浓度）、杀菌试验和结晶紫染色法，测定了新型抗菌肽CSA - 13、CSA - 44和CSA - 131（天然抗菌肽的合成模拟物）与头孢他啶、左氧氟沙星、复方新诺明和黏菌素联合给药对（某菌名未给出）临床菌株的益处（潜在协同作用）和抗生物膜活性。
结果
获得的数据表明，新型抗菌肽对浮游培养和静态生物膜中生长的受试（某菌名未给出）菌株具有很强的活性。此外，在一些菌株中，还观察到新型抗菌肽与传统抗生素的协同作用。结论：我们的数据表明，新型抗菌肽是未来开发治疗（某菌名未给出）感染新方法的有前景的药物，同时其有可能与传统抗生素联合使用。
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