环状RNA-RANGAP1/微小RNA-542-3p/肌球蛋白调节轻链相互作用蛋白轴调控骨肉瘤细胞进展。

circ-RANGAP1/MicroRNA-542-3p/Myosin Regulatory Light Chain Interacting Protein Axis Modulates the Osteosarcoma Cell Progression.

作者信息

Sheng Jundong, Liu Jin, Du Junwang, Wang Yongping

机构信息

Department of Orthopedics, First People's Hospital of Tianshui, Tianshui, 741000 Gansu, China.

Department of Anesthesiology, First People's Hospital of Tianshui, Tianshui, 741000 Gansu, China.

出版信息

Appl Bionics Biomech. 2022 Jun 14;2022:4247670. doi: 10.1155/2022/4247670. eCollection 2022.

DOI:10.1155/2022/4247670

PMID:35747400

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9213143/

Abstract

OBJECTIVE

This study is aimed at exploring the influence of circular RNA- (circRNA-) RANGAP1 targeting microRNA- (miR-) 542-3p/myosin regulatory light chain interacting protein (MYLIP) on the biological function of osteosarcoma (OS) cells.

METHODS

Tumor tissues and normal tissues were collected from OS patients and circ-RANGAP1, miR-542-3p, and MYLIP expression was tested by RT-qPCR. The correlation between the clinicopathology/prognosis of patients with OS and circ-RANGAP1 expression was observed. Human OS cell line MG-63 was screened to determine the influences of circ-RANGAP1 and miR-542-3p on OS cell progression. The targeting relation of circ-RANGAP1, miR-542-3p, and MYLIP was probed.

RESULTS

circ-RANGAP1 expression was elevated in tumor tissues from OS patients, which was correlated to the poor clinicopathology. circ-RANGAP1 expression was augmented in males or patients younger than 20 years old or patients with advanced OS. Higher circ-RANGAP1 expression indicated a poor prognosis in OS patients. After silencing circ-RANGAP1 or elevating miR-542-3p in MG63 cells, cell progression was limited. miR-542-3p downregulation reduced the therapeutic efficacy of silenced circ-RANGAP1. circ-RANGAP1 bound with miR-542-3p to target MYLIP.

CONCLUSION

Silenced circ-RANGAP1 boosts MYLIP expression via competitive binding of miR-542-3p to facilitate OS cell progression.

摘要

目的

本研究旨在探讨环状RNA-（circRNA-）RANGAP1靶向微小RNA-（miR-）542-3p/肌球蛋白调节轻链相互作用蛋白（MYLIP）对骨肉瘤（OS）细胞生物学功能的影响。

方法

收集骨肉瘤患者的肿瘤组织和正常组织，采用RT-qPCR检测circ-RANGAP1、miR-542-3p和MYLIP的表达。观察骨肉瘤患者临床病理/预后与circ-RANGAP1表达的相关性。筛选人骨肉瘤细胞系MG-63，确定circ-RANGAP1和miR-542-3p对骨肉瘤细胞进展的影响。探究circ-RANGAP1、miR-542-3p和MYLIP之间的靶向关系。

结果

骨肉瘤患者肿瘤组织中circ-RANGAP1表达升高，与临床病理不良相关。男性、20岁以下患者或晚期骨肉瘤患者的circ-RANGAP1表达增加。circ-RANGAP1表达较高表明骨肉瘤患者预后较差。在MG63细胞中沉默circ-RANGAP1或提高miR-542-3p水平后，细胞进展受到限制。miR-542-3p下调降低了沉默circ-RANGAP1的治疗效果。circ-RANGAP1与miR-542-3p结合靶向MYLIP。