Gynecological Department, Wuhan Hanyang Hospital, Wuhan, Hubei, China.
Department of Oncology, Chengdu Fifth People's Hospital, Chengdu, Sichuan, China.
Cancer Biomark. 2021;30(3):287-298. doi: 10.3233/CBM-201523.
The dysregulation of microRNA-802 (miR-802) has crucial roles in cancer progression. Nevertheless, the bio-function of miR-802 in cervical cancer remains unclear.
Hence, we illuminated the potential roles of miR-802 in cervical cancer cell growth, migration, and invasion.
The levels of miR-802 and myosin regulatory light chain interacting protein (MYLIP) were measured using qRT-PCR assay. The potential effects of miRNA-802 on cervical cancer cell proliferation and metastatic phenotypes were determined using CCK-8, colony formation, wound healing and Transwell invasion assays. MYLIP was validated as a downstream target gene of miRNA-802 using bioinformatics analysis tool and luciferase report gene assay. The impact of miR-802 on the growth of cervical cancer cell in vivo was analyzed using xenograft model. The expression of MYLIP was measured by western blotting and immunohistochemistry (IHC).
MiRNA-802 was distinctly down-regulated in cervical cancer cells as well as clinical cervical cancer samples. Upregulation of miRNA-802 significantly inhibited the growth and aggressiveness of cervical cancer cell. Additional, MYLIP was a functional target of miR-802. MYLIP was ovrerexpressed in cervical cancer and MYLIP level was negatively associated with the level of miR-802. Overexpression of MYLIP eliminated the inhibitory effects of miR-802 on growth and metastatic-related traits of cervical cancer cell. In vivo, miR-802 also markedly reduced the tumor growth of cervical cancer cell and decreased the expression of MYLIP.
MiR-802 inhibits the growth and metastatic-related phenotypes of cervical cancer cell through targeting MYLIP.
微小 RNA-802（miR-802）的失调在癌症进展中起着关键作用。然而，miR-802 在宫颈癌中的生物学功能尚不清楚。
因此，我们阐明了 miR-802 在宫颈癌细胞生长、迁移和侵袭中的潜在作用。
使用 qRT-PCR 测定 miR-802 和肌球蛋白调节轻链相互作用蛋白（MYLIP）的水平。使用 CCK-8、集落形成、划痕愈合和 Transwell 侵袭测定来确定 miRNA-802 对宫颈癌细胞增殖和转移表型的潜在影响。使用生物信息学分析工具和荧光素酶报告基因测定验证 MYLIP 是 miRNA-802 的下游靶基因。使用异种移植模型分析 miR-802 对宫颈癌细胞体内生长的影响。通过 Western blot 和免疫组织化学（IHC）测定 MYLIP 的表达。
miR-802 在宫颈癌细胞和临床宫颈癌样本中明显下调。miR-802 的上调显著抑制了宫颈癌细胞的生长和侵袭能力。此外，MYLIP 是 miR-802 的功能靶基因。MYLIP 在宫颈癌中过表达，且 MYLIP 水平与 miR-802 水平呈负相关。过表达 MYLIP 消除了 miR-802 对宫颈癌细胞生长和转移相关特征的抑制作用。在体内，miR-802 还显著降低了宫颈癌细胞的肿瘤生长并降低了 MYLIP 的表达。
miR-802 通过靶向 MYLIP 抑制宫颈癌细胞的生长和转移相关表型。
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