Department of Orthopedics, Dongying Traditional Chinese Medicine Hospital, Dongying, 257055 Shandong, China.
Department of Clinical Laboratory, Dongying Traditional Chinese Medicine Hospital, Dongying, 257055 Shandong, China.
Anal Cell Pathol (Amst). 2021 Jul 7;2021:5525711. doi: 10.1155/2021/5525711. eCollection 2021.
Osteosarcoma (OS) is a kind of fatal primary bone tumors in adolescents and young adults. Long noncoding RNAs (lncRNAs) are a group of noncoding RNAs which occupy a part of the latest hot topics. We aimed to investigate the roles of lncRNA LINC00665 in OS in this study. In this study, we found that LINC00665 was highly expressed in OS tissues and cell lines, and its high expression was associated with malignant feature and poor prognosis of OS. In OS cells, LINC00665 could facilitate the proliferation, migration, and invasion to play an oncogenic role. Mechanistically, LINC00665 served as a sponge for miR-708 and miR-142-5p and positively mediated the expression of their target RAP1B. Finally, we confirmed that LINC00665 exercised its biological functions by mediating RAP1B. In conclusion, LINC00665 is overexpressed in OS and facilitates the malignant processes of OS cells by increasing the RAP1B expression via sponging miR-708 and miR-142-5p.
骨肉瘤（OS）是一种发生于青少年和年轻成人的致命原发性骨肿瘤。长链非编码 RNA（lncRNA）是一组非编码 RNA，占据了最新热点话题的一部分。在本研究中，我们旨在探究 lncRNA LINC00665 在 OS 中的作用。本研究发现，LINC00665 在 OS 组织和细胞系中呈高表达，其高表达与 OS 的恶性特征和不良预后相关。在 OS 细胞中，LINC00665 可促进增殖、迁移和侵袭，发挥致癌作用。在机制上，LINC00665 作为 miR-708 和 miR-142-5p 的海绵分子，正向调节其靶基因 RAP1B 的表达。最后，我们证实 LINC00665 通过介导 RAP1B 发挥其生物学功能。总之，LINC00665 在 OS 中呈过表达，通过海绵吸附 miR-708 和 miR-142-5p 增加 RAP1B 的表达，从而促进 OS 细胞的恶性进程。
