大鼠基底神经节中蛋氨酸脑啡肽、亮氨酸脑啡肽和神经降压素浓度随年龄增长而降低。

Age-related decreases in the concentration of Met- and Leu-enkephalin and neurotensin in the basal ganglia of rats.

作者信息

De Ceballos M L, Boyce S, Taylor M, Jenner P, Marsden C D

出版信息

Neurosci Lett. 1987 Mar 20;75(1):113-7. doi: 10.1016/0304-3940(87)90085-1.

Abstract

Previous studies using radioimmunoassay procedures have failed to show age-related changes in the concentration of Met- and Leu-enkephalin or neurotensin in rat basal ganglia. In contrast, using a combined high-pressure liquid chromatography (HPLC)-radioimmunoassay (RIA) technique we now report considerable decreases in the levels of these neuropeptides in areas of basal ganglia of 22 months old-compared to 3 months-old male Wistar rats. The concentration of Met-enkephalin was greatly reduced in the striatum and nucleus accumbens, but not in substantia nigra, of old compared to young animals. There was a similarly large decrease in Leu-enkephalin content in striatum of old rats with less marked decreases occurring in both the nucleus accumbens and substantia nigra. Neurotensin levels in the striatum and substantia nigra were greatly reduced in old rats, with a less marked decrease in the nucleus accumbens.

摘要

以往采用放射免疫分析方法的研究未能显示大鼠基底神经节中甲硫氨酸脑啡肽、亮氨酸脑啡肽或神经降压素浓度的年龄相关变化。相比之下，我们现在采用高压液相色谱（HPLC）与放射免疫分析（RIA）相结合的技术报告称，与3个月大的雄性Wistar大鼠相比，22个月大的大鼠基底神经节区域中这些神经肽的水平大幅下降。与年轻动物相比，老年动物纹状体和伏隔核中甲硫氨酸脑啡肽的浓度大幅降低，但黑质中未降低。老年大鼠纹状体中亮氨酸脑啡肽含量也有类似的大幅下降，伏隔核和黑质中的下降不太明显。老年大鼠纹状体和黑质中的神经降压素水平大幅降低，伏隔核中的下降不太明显。