College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Key Laboratory of Forest Ecosystem Process and Management of Fujian Province, Fuzhou, 350002, China.
BMC Plant Biol. 2022 Jun 24;22(1):307. doi: 10.1186/s12870-022-03680-z.
Development of Pantana phyllostachysae, a moso bamboo pest, is affected by its diet. Understanding the mechanism underlying the different insect-resistant capacities of on- and off-year moso bamboo fed by P. phyllostachysae is crucial for managing pest outbreaks. As microbes were proven to influence plant immunity, we compared gut microbial communities of P. phyllostachysae with different diets by metabarcoding sequencing. By using sterilization assay, microbes were removed from leaf surfaces, and thus we confirmed that microbes inhabiting moso bamboo leaves impact the weight of P. phyllostachysae larva. Furthermore, the gut microbial communities of P. phyllostachysae fed on on- and off-year bamboo leaves were compared, to identify the functional microbial communities that impact the interaction between bamboo leaves and P. phyllostachysae.
We found that species from orders Lactobacillales and Rickettsiales are most effective within functional microbiota. Functional prediction revealed that gut microbes of larva fed on on-year leaves were related to naphthalene degradation, while those fed on off-year leaves were related to biosynthesis of ansamycins, polyketide sugar unit biosynthesis, metabolism of xenobiotics, and tetracycline biosynthesis. Most functional microbes are beneficial to the development of larva that feed on on-year bamboo leaves, but damage the balance of intestinal microenvironment and immune systems of those larva that feed on off-year leaves.
This work developed an efficient strategy for microbiome research of Lepidopteran insects and provided insights into microbiota related to the interaction between host plants and P. phyllostachysae. We provided microbial candidates for the ecological control of P. phyllostachysae according to the function of effective microbiota.
毛竹长足象的发育受其食物的影响。了解毛竹长足象对大年竹和小年竹的不同抗虫能力的机制对于管理虫害爆发至关重要。由于微生物被证明会影响植物的免疫力，我们通过宏条形码测序比较了不同食物来源的毛竹长足象的肠道微生物群落。通过使用灭菌实验，我们从叶片表面去除微生物，从而证实了栖息在毛竹叶片上的微生物会影响毛竹长足象幼虫的体重。此外，我们比较了取食大年竹和小年竹叶片的毛竹长足象的肠道微生物群落，以鉴定影响竹叶片与毛竹长足象相互作用的功能微生物群落。
我们发现乳杆菌目和立克次氏体目在功能微生物群中最有效。功能预测显示，取食大年竹叶片的幼虫肠道微生物与萘降解有关，而取食小年竹叶片的幼虫肠道微生物与安莎霉素生物合成、聚酮糖单元生物合成、外来化合物代谢和四环素生物合成有关。大多数功能微生物对取食大年竹叶片的幼虫的发育有益，但会破坏取食小年竹叶片的幼虫肠道微环境和免疫系统的平衡。
这项工作为鳞翅目昆虫的微生物组研究开发了一种有效的策略，并为宿主植物与毛竹长足象相互作用相关的微生物群提供了新的见解。根据有效微生物群的功能，我们为毛竹长足象的生态控制提供了微生物候选物。
