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芪参益气滴丸通过抑制大鼠PI3K-AKT信号通路保护糖尿病肾病。

Qishen Yiqi Dripping Pill Protects Diabetic Nephropathy by Inhibiting the PI3K-AKT Signaling Pathways in Rats.

作者信息

Li Shaohua, Xu Guangbiao

机构信息

Department of Nephrology, The First Affiliated Hospital (Southwest Hospital) of Army Medical University, Chongqing 400038, China.

Department of Nephrology, Taizhou Hospital of Zhejiang Province, Taizhou 317000, China.

出版信息

Evid Based Complement Alternat Med. 2022 Jun 17;2022:6239829. doi: 10.1155/2022/6239829. eCollection 2022.

DOI:10.1155/2022/6239829
PMID:35754685
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9232344/
Abstract

This study aimed to explore the potential mechanisms of Qishen Yiqi dripping pill (QYDP) against diabetic nephropathy (DN) through network pharmacology and animal experiments. The components and targets of QYDP and DN-related targets were retrieved from public databases. A total of 116 compounds and 160 targets of QYDP anti-DN were obtained. The top 10 hub targets including AKT1, TNF, ALB, INS, PPARG, IL-6, IL-1B, VEGF-A, JUN, and MAPK3 were screened by Cytoscape software. Then, the key targets of QYDP were enriched in 1815 Gene Ontology (GO) entries ( < 0.01) and 159 Kyoto Encyclopedia of Genomes and Genomes (KEGG) pathways ( < 0.01), mainly including the AGE-RAGE signaling pathway in diabetic complications and the PI3K-AKT signaling pathway. In animal experiments, the results of an ELISA assay showed that QYDP could regulate the expression levels of kidney function-related indexes and reduce the expression of inflammatory factors. qRT-PCR and western blot results showed that QYDP regulated the expression of hub genes. In conclusion, this study shows that QYDP could treat DN by antioxidative and antiinflammatory activity and inhibiting the PI3K-AKT signaling pathway.

摘要

本研究旨在通过网络药理学和动物实验探索芪参益气滴丸（QYDP）抗糖尿病肾病（DN）的潜在机制。从公共数据库中检索QYDP的成分和靶点以及DN相关靶点。共获得116种QYDP抗DN的化合物和160个靶点。通过Cytoscape软件筛选出前10个核心靶点，包括AKT1、TNF、ALB、INS、PPARG、IL-6、IL-1B、VEGF-A、JUN和MAPK3。然后，QYDP的关键靶点富集在1815个基因本体论（GO）条目（<0.01）和159条京都基因与基因组百科全书（KEGG）通路（<0.01）中，主要包括糖尿病并发症中的AGE-RAGE信号通路和PI3K-AKT信号通路。在动物实验中，ELISA检测结果表明QYDP可调节肾功能相关指标的表达水平并降低炎症因子的表达。qRT-PCR和western blot结果表明QYDP可调节核心基因的表达。总之，本研究表明QYDP可通过抗氧化和抗炎活性以及抑制PI3K-AKT信号通路来治疗DN。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a4f/9232344/7ee1a0bd9872/ECAM2022-6239829.009.jpg
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Front Pharmacol. 2022 Jan 5;12:792777. doi: 10.3389/fphar.2021.792777. eCollection 2021.
2
Proanthocyanidins Protect Against Cadmium-Induced Diabetic Nephropathy Through p38 MAPK and Keap1/Nrf2 Signaling Pathways.原花青素通过p38丝裂原活化蛋白激酶和Keap1/Nrf2信号通路预防镉诱导的糖尿病肾病。
Front Pharmacol. 2022 Jan 3;12:801048. doi: 10.3389/fphar.2021.801048. eCollection 2021.
3
IDF Diabetes Atlas: Global, regional and country-level diabetes prevalence estimates for 2021 and projections for 2045.
电针调节P2XR-NLRP3炎症级联反应以缓解2型糖尿病干眼大鼠眼表感觉减退。
Purinergic Signal. 2024 Mar 11. doi: 10.1007/s11302-024-09991-0.
国际糖尿病联盟（IDF）糖尿病地图集：2021 年全球、区域和国家糖尿病患病率估算值以及 2045 年预测值。
Diabetes Res Clin Pract. 2022 Jan;183:109119. doi: 10.1016/j.diabres.2021.109119. Epub 2021 Dec 6.
4
Dioscin protects against diabetic nephropathy by inhibiting renal inflammation through TLR4/NF-κB pathway in mice.薯蓣皂苷通过抑制 TLR4/NF-κB 通路减轻糖尿病肾病小鼠肾脏炎症反应。
Immunobiology. 2020 May;225(3):151941. doi: 10.1016/j.imbio.2020.151941. Epub 2020 Apr 19.
5
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