Ambrose Luke, Popovic Iva, Hereward James, Ortiz-Barrientos Daniel, Beebe Nigel W
School of Biological Sciences, University of Queensland, Brisbane, Australia.
CSIRO, Brisbane, QLD, Australia.
iScience. 2022 Jun 3;25(7):104521. doi: 10.1016/j.isci.2022.104521. eCollection 2022 Jul 15.
We investigate the genetic basis of anthropophily (human host use) in a non-model mosquito species group, the complex from the southwest Pacific. This complex has experienced multiple transitions from anthropophily to zoophily, contrasting with well-studied systems (the global species and the African complex) that have evolved to be specialist anthropophiles. By performing tests of selection and assessing evolutionary patterns for >200 olfactory genes from nine genomes, we identify several candidate genes associated with differences in anthropophily in this complex. Based on evolutionary patterns (phylogenetic relationships, fixed amino acid differences, and structural differences) as well as results from selection analyses, we identify numerous genes that are likely to play an important role in mosquitoes' ability to detect humans as hosts. Our findings contribute to the understanding of the evolution of insect olfactory gene families and mosquito host preference as well as having potential applied outcomes.
我们研究了一个非模式蚊种群体——来自西南太平洋的复合体中嗜人习性（以人类为宿主）的遗传基础。该复合体经历了多次从嗜人习性向嗜动物习性的转变，这与经过充分研究的系统（全球物种和非洲复合体）形成对比，后者已进化成为专门的嗜人者。通过对来自九个基因组的200多个嗅觉基因进行选择测试并评估进化模式，我们在这个复合体中确定了几个与嗜人习性差异相关的候选基因。基于进化模式（系统发育关系、固定氨基酸差异和结构差异）以及选择分析结果，我们确定了许多可能在蚊子将人类识别为宿主的能力中发挥重要作用的基因。我们的研究结果有助于理解昆虫嗅觉基因家族的进化以及蚊子的宿主偏好，同时也具有潜在的应用成果。
