Salman Baher I, Ibrahim Adel Ehab, El Deeb Sami, Saraya Roshdy E
Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University Assiut Branch Assiut 71524 Egypt
Natural and Medical Sciences Research Center, University of Nizwa Birket Almawz P. O. box 33 Oman.
RSC Adv. 2022 Jun 6;12(26):16624-16631. doi: 10.1039/d2ra02241a. eCollection 2022 Jun 1.
The COVID-19 pandemic has encouraged the search for novel antiviral medications. Recently, molnupiravir (MOL) has been approved as an oral antiviral to manage COVID-19. Thus, the development of sensitive and cost-effective methods for quantification of MOL in real plasma samples (pharmacokinetic) and pharmaceutical tablets is required. Herein, we present the fabrication of novel fluorescent polyamine quantum dots (PA@CQDs) fabricated from apricots using one step synthesis for analysis of MOL. The relative fluorescence intensity (RFI) of the synthesized quantum dots was influentially quenched by the addition of molnupiraivr. The linear range was found to be between 2-70 ng mL with lower limit of quantitation (LOQ) equal to 1.61 ng mL. The fluorescent probe was successfully utilized in a pharmacokinetic study of MOL with maximum plasma concentration ( ) 920.2 ± 6.12 ng mL without any matrix interference. The sensitivity and selectivity of the presented method allow its application in clinical laboratories.
新冠疫情促使人们寻找新型抗病毒药物。最近，莫努匹拉韦（MOL）已被批准作为一种口服抗病毒药物用于治疗新冠。因此，需要开发灵敏且经济高效的方法来定量分析实际血浆样本（药代动力学）和药物片剂中的莫努匹拉韦。在此，我们展示了通过一步合成法由杏子制备新型荧光多胺量子点（PA@CQDs）用于莫努匹拉韦分析。添加莫努匹拉韦会显著淬灭合成量子点的相对荧光强度（RFI）。线性范围为2 - 70 ng/mL，定量下限（LOQ）等于1.61 ng/mL。该荧光探针成功用于莫努匹拉韦的药代动力学研究，最大血浆浓度（ ）为920.2 ± 6.12 ng/mL，且无任何基质干扰。所提出方法的灵敏度和选择性使其可应用于临床实验室。
