Zhang Jialei, Zhang Xiaoling, Yang Yongyan, Zhao Jun, Hu Wenqing, Yu Yonghao
Department of Anesthesiology, Tianjin Medical University General Hospital, Tianjin, China.
Department of Anesthesiology, Changzhi People's Hospital Affiliated to Changzhi Medical College, Changzhi, China.
Front Aging Neurosci. 2022 Jun 10;14:940106. doi: 10.3389/fnagi.2022.940106. eCollection 2022.
Although the biological relationship between vitamin D (VD) deficiency and cognitive function has been recognized by many scholars, the theoretical mechanisms involved are still not well-understood. In this study, we demonstrated the role of VD in alleviating the cognitive dysfunction in aged mice caused by sevoflurane anesthesia. Forty female C57BL/6 mice aged 12 months were selected for the experiment. VD (-) and VD (+) mouse models and sevoflurane anesthesia models were established. Mice were randomly divided into normal elderly group (NC group), normal aged mice + sevoflurane anesthesia treatment group (NS group), aged VD (-) mice + sevoflurane anesthesia treatment group [VD (-) group], and aged VD (+) + sevoflurane anesthesia treatment group [VD (+) group]. To compare the emergence time after sevoflurane anesthesia in aged mice with different levels of VD and to test the cognitive function of four groups through the water maze. Inflammatory factor expression and cholinergic activity in hippocampus tissue of all mice were measured at the end of behavioral tests. These data show that, low levels of VD aggravated the delayed emergence and cognitive dysfunction in aged mice caused by sevoflurane anesthesia, while higher levels of VD mitigated this impairment by enhancing cholinergic activity and reducing inflammatory factor expression in the hippocampus.
尽管维生素D（VD）缺乏与认知功能之间的生物学关系已被许多学者所认识，但其涉及的理论机制仍未得到充分理解。在本研究中，我们证明了VD在减轻七氟醚麻醉所致老年小鼠认知功能障碍中的作用。选取40只12月龄雌性C57BL/6小鼠进行实验。建立VD（-）和VD（+）小鼠模型以及七氟醚麻醉模型。将小鼠随机分为正常老年组（NC组）、正常老年小鼠+七氟醚麻醉治疗组（NS组）、老年VD（-）小鼠+七氟醚麻醉治疗组[VD（-）组]和老年VD（+）+七氟醚麻醉治疗组[VD（+）组]。比较不同VD水平老年小鼠七氟醚麻醉后的苏醒时间，并通过水迷宫测试四组小鼠的认知功能。在行为测试结束时，检测所有小鼠海马组织中的炎症因子表达和胆碱能活性。这些数据表明，低水平的VD加重了七氟醚麻醉所致老年小鼠的苏醒延迟和认知功能障碍，而高水平的VD通过增强胆碱能活性和降低海马中的炎症因子表达减轻了这种损伤。
