Morelli L, Cocconcelli P S, Bottazzi V, Damiani G, Ferretti L, Sgaramella V
Plasmid. 1987 Jan;17(1):73-5. doi: 10.1016/0147-619x(87)90013-8.
A method for the transformation of Lactobacillus protoplasts by plasmid DNA is reported. The procedure involves polyethylene glycol treatment of protoplasts to induce DNA uptake. A transformation efficiency ranging from 5 to 1000 transformants per microgram of DNA is achieved; the efficiency of protoplast regeneration ranged from 10 to 20%.
