Mitochondrial-Targeted Ratiometric Fluorescent Probe to Monitor ClO Induced by Ferroptosis in Living Cells.

Ferroptosis is a type of iron-dependent programmed cell death. Once such kind of death occurs, an individual cell would undergo a series of changes related to reactive oxygen species (ROS) in mitochondria. A mitochondrial-targeted ratiometric fluorescent probe () was developed to specifically detect ferroptosis-induced ClO, whose recognition group is -methoxyphenol, and the mitochondrial-targeted group is benzimidazole. The fluorescence of was first quenched by 30 μM of Fe, and then appeared as a ratiometric signal at 477 nm and 392 nm in response to ferroptosis-induced ClO in living cells. was successfully used to evaluate changes in ClO induced by ferroptosis.