Department of Stomatology, Taizhou Central Hospital (Taizhou University Hospital), Taizhou, 318000 Zhejiang Province, China.
Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, 230000 Anhui Province, China.
Comput Math Methods Med. 2022 Jun 17;2022:2857022. doi: 10.1155/2022/2857022. eCollection 2022.
Oral squamous cell carcinoma (OSCC) has increasingly become a worldwide health concern, and its survival rate has not been much improved partially due to a deficiency of precise molecular markers. Dysregulation of LINC01116, a long noncoding RNA sequence, has been observed in several types of cancer. However, the role played by LINC01116 in OSCC has not yet been fully elaborated. This study explored how LINC01116 was involved in the regulation of OSCC progression by analyzing expressions of LINC01116 in OSCC patients. The findings demonstrated upregulation of LINC01116 in OSCC tissues as opposed to regular oral mucosa, and overexpression of LINC01116 was correlated with advanced tumor status. LINC01116 knockdown using shRNA markedly reduced the OSCC cell invasion and migration in vitro. Moreover, the expression of LINC01116 was negatively correlated with that of microRNA-9-5p (miR-9). Luciferase reporter and loss-of-function assays demonstrated that LINC01116 functioned as a competing endogenous RNA (ceRNA) that could effectively sponge miR-9, thus regulating the derepression of matrix metalloproteinase 1 (MMP1). Furthermore, we confirmed that LINC01116 knockdown did not affect the expression of MMP1 messenger RNA (mRNA). Collectively, it is demonstrated in this study that overexpression of LINC01116 can promote the OSCC progression. The LINC01116-miR-9-MMP1 axis provides a novel insight into the OSCC pathogenesis and offers potential therapeutic targets against OSCC.
口腔鳞状细胞癌 (OSCC) 已成为全球关注的健康问题，其生存率并未得到显著提高，部分原因是缺乏精确的分子标志物。长链非编码 RNA 序列 LINC01116 的失调已在多种类型的癌症中观察到。然而，LINC01116 在 OSCC 中的作用尚未得到充分阐述。本研究通过分析 OSCC 患者中 LINC01116 的表达，探讨了 LINC01116 如何参与 OSCC 进展的调控。研究结果表明，LINC01116 在 OSCC 组织中呈上调表达，与正常口腔黏膜相反，并且 LINC01116 的过表达与肿瘤的晚期状态相关。使用 shRNA 敲低 LINC01116 可显著减少 OSCC 细胞的体外侵袭和迁移。此外，LINC01116 的表达与 microRNA-9-5p (miR-9) 的表达呈负相关。荧光素酶报告基因和功能丧失实验表明，LINC01116 作为竞争性内源 RNA (ceRNA) 发挥作用，可以有效地吸附 miR-9，从而调节基质金属蛋白酶 1 (MMP1) 的去抑制。此外，我们证实 LINC01116 敲低不影响 MMP1 信使 RNA (mRNA) 的表达。综上所述，本研究表明 LINC01116 的过表达可促进 OSCC 的进展。LINC01116-miR-9-MMP1 轴为 OSCC 的发病机制提供了新的见解，并为 OSCC 的治疗提供了潜在的靶点。
