Yin Genshen, Yang Man, Chen Ye, Xi Huihui, Jiang Mingxing
School of Agriculture and Life Sciences, Kunming University, Kunming, Yunnan Province, China.
School of Life Sciences, Zhengzhou University, Zhengzhou, Henan Province, China.
Mitochondrial DNA B Resour. 2022 Jun 20;7(6):1081-1083. doi: 10.1080/23802359.2022.2086494. eCollection 2022.
var. (W. W. Smith) Stearn 1948 is an ornamental and medicinal plant that has an extremely limited distribution in Yunnan, China. Here, we obtained the complete plastome of . var. via next-generation sequencing, and conducted phylogenomic analyses with existing species from . The total length of . var. was 152,206 bp with a typical quadripartite structure. The whole plastome contained a pair of inverted repeats (IRa/IRb; 26,399 bp) which divided a large single-copy (LSC; 81,854 bp) and a small single-copy (SSC; 17,563 bp). The average GC content among the whole plastome sequence and the LSC, SSC, and IR regions were 37%, 34.8%, 30.6%, and 42.5%, respectively. There were 134 genes detected from the whole plastome sequence, including 87 protein-coding genes, 39 tRNAs, and 8 rRNAs. Phylogenetic analyses using maximum likelihood showed congruent results that . var together with var. formed a single branch. These results demonstrate a close relationship between these variation species. The newly characterized chloroplast genome presented here will provide essential data for further phylogenomic analyses of the intraspecific relationship among species and for conservation genetics research of var. .
变种（W. W. 史密斯）斯特恩1948年记载的是一种观赏兼药用植物，在中国云南的分布极为有限。在此，我们通过二代测序获得了该变种的完整叶绿体基因组，并与该属现有的物种进行了系统基因组分析。该变种的叶绿体基因组全长152,206 bp，具有典型的四分体结构。整个叶绿体基因组包含一对反向重复序列（IRa/IRb；26,399 bp），将一个大单拷贝区（LSC；81,854 bp）和一个小单拷贝区（SSC；17,563 bp）分开。整个叶绿体基因组序列以及LSC、SSC和IR区域的平均GC含量分别为37%、34.8%、30.6%和42.5%。从整个叶绿体基因组序列中检测到134个基因，包括87个蛋白质编码基因、39个tRNA和8个rRNA。使用最大似然法进行的系统发育分析显示了一致的结果，即该变种与另一变种形成了一个单独的分支。这些结果表明这些变种之间关系密切。这里新鉴定的叶绿体基因组将为该属物种种内关系的进一步系统基因组分析以及该变种的保护遗传学研究提供重要数据。
