Synzynys B I, Aminev A G, Saenko A S, Pelevina I I
Radiobiologiia. 1987 Mar-Apr;27(2):224-7.
A gamma-radiation dose (Di) suppressing DNA synthesis initiation by 35% in primary suspension cultures of mammalian cells, is nearly the same as D0 for survival of clonogenic cells of the same lines and tissues. The extent of DNA synthesis suppression is assessed by impulse 3H-thymidine incorporation in the acid-insoluble fraction of irradiated cells. The values of Di determined in this way for HeLa cells, Ehrlich ascites tumor cells, mouse bone marrow and thymus cells are 2.0, 1.5, 1.5, and 1.0 Gy, respectively; as determined by clonogenic capacity of these cells, Di = 1.9, 2.0, 1.3, and 1.0 Gy, respectively.
在哺乳动物细胞原代悬浮培养物中，抑制DNA合成起始35%的γ辐射剂量（Di），与同细胞系和组织的克隆形成细胞存活的D0剂量几乎相同。DNA合成抑制程度通过脉冲3H-胸腺嘧啶核苷掺入受辐照细胞的酸不溶性部分来评估。以这种方式测定的HeLa细胞、艾氏腹水瘤细胞、小鼠骨髓和胸腺细胞的Di值分别为2.0、1.5、1.5和1.0 Gy；根据这些细胞的克隆形成能力测定，Di分别为1.9、2.0、1.3和1.0 Gy。
