Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, PR China.
Tianjin Agricultural University, Tianjin 300384, PR China; State Key Laboratory of Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, PR China.
Int J Biol Macromol. 2020 Nov 15;163:1640-1648. doi: 10.1016/j.ijbiomac.2020.09.068. Epub 2020 Sep 14.
The polysaccharides (CSPw, CSPc, CSPa, and CSPu) were prepared by hot water extraction, acid-assisted extraction, alkaline-assisted extraction, and ultrasound-assisted extraction from corn silk, respectively. High performance gel permeation chromatography (HPGPC), fourier-transform infrared (FT-IR) spectroscopy, and scanning electron microscopy (SEM) results indicated that the extraction methods had an obvious impact on the molecular weight, structure, and morphology of the CSPs. Among the four polysaccharides, CSPu showed the highest inhibitory α-glucosidase activity, which might be related to its smaller molecular weight. Furthermore, kinetics analyses revealed that CSPu had significant inhibition of α-glucosidase in a non-reversible and competitive manner. Fluorescence quenching analysis illustrated that the interaction mechanism of CSPu and α-glucosidase was claimed as a static quenching mechanism. Isothermal titration calorimetry (ITC) analysis showed that the main driving forces for the interaction of CSPu with α-glucosidase was hydrogen bonding and the binding interactions of them occurred spontaneously.
玉米须中的多糖（CSPw、CSPc、CSPa 和 CSPu）分别通过热水提取、酸辅助提取、碱辅助提取和超声辅助提取制备。高效凝胶渗透色谱（HPGPC）、傅里叶变换红外（FT-IR）光谱和扫描电子显微镜（SEM）结果表明，提取方法对 CSPs 的分子量、结构和形态有明显影响。在四种多糖中，CSPu 表现出最高的抑制 α-葡萄糖苷酶活性，这可能与其较小的分子量有关。此外，动力学分析表明 CSPu 以非可逆和竞争性方式对 α-葡萄糖苷酶有显著抑制作用。荧光猝灭分析表明，CSPu 与 α-葡萄糖苷酶的相互作用机制为静态猝灭机制。等温滴定量热法（ITC）分析表明，CSPu 与 α-葡萄糖苷酶相互作用的主要驱动力是氢键，它们的结合相互作用是自发发生的。
