新型氧化海藻酸钠-明胶-丝素蛋白生物墨水的配方与表征及其在皮肤再生中的应用。
Formulation and Characterization of a Novel Oxidized Alginate-Gelatin-Silk Fibroin Bioink with the Aim of Skin Regeneration.
机构信息
Department of Nanotechnology and Advanced Materials, Materials and Energy Research Center, Karaj, Iran.
Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran, Iran.
出版信息
Iran Biomed J. 2023 Sep 1;27(5):280-93. doi: 10.61186/ibj.27.5.280. Epub 2023 Aug 23.
BACKGROUND
In the present study, a novel bioink was suggested based on the oxidized alginate (OAlg), gelatin (GL), and silk fibroin (SF) hydrogels.
METHODS
The composition of the bioink was optimized by the rheological and printability measurements, and the extrusion-based 3D bioprinting process was performed by applying the optimum OAlg-based bioink.
RESULTS
The results demonstrated that the viscosity of bioink was continuously decreased by increasing the SF/GL ratio, and the bioink displayed a maximum achievable printability (92 ± 2%) at 2% (w/v) of SF and 4% (w/v) of GL. Moreover, the cellular behavior of the scaffolds investigated by MTT assay and live/dead staining confirmed the biocompatibility of the prepared bioink.
CONCLUSION
The bioprinted OAlg-GL-SF scaffold could have the potential for using in skin tissue engineering applications, which needs further exploration.
背景
在本研究中，提出了一种基于氧化海藻酸钠（OAlg）、明胶（GL）和丝素蛋白（SF）水凝胶的新型生物墨水。
方法
通过流变学和可打印性测量优化生物墨水的组成，并通过应用最佳的 OAlg 基生物墨水进行基于挤出的 3D 生物打印过程。
结果
结果表明，生物墨水的粘度通过增加 SF/GL 比连续降低，并且在 SF 为 2%（w/v）和 GL 为 4%（w/v）时，生物墨水显示出最大可实现的可打印性（92±2%）。此外，通过 MTT 测定和活/死染色研究支架的细胞行为证实了制备的生物墨水的生物相容性。
结论
OAlg-GL-SF 支架的生物打印具有用于皮肤组织工程应用的潜力，这需要进一步探索。
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