具有潜在骨组织工程应用的双交联原位形成的海藻酸钠/丝素水凝胶。
Dual-crosslinked in-situ forming alginate/silk fibroin hydrogel with potential for bone tissue engineering.
机构信息
Biomedical Engineering Division, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran.
Biomedical Engineering Division, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran.
出版信息
Biomater Adv. 2023 Oct;153:213565. doi: 10.1016/j.bioadv.2023.213565. Epub 2023 Jul 27.
This study aimed to improve the mechanical and biological properties of alginate-based hydrogels. For this purpose, in-situ forming hydrogels were prepared by dual crosslinking of Alginate (Alg)/Oxidized Alginate (OAlg)/Silk Fibroin (SF) through simultaneous ionic gelation using CaCO-GDL and Schiff-base reaction. The resulting hydrogels were characterized by FTIR, SEM, compressive modulus, and rheological tests. Compared to the physically-crosslinked alginate hydrogel, the compressive modulus of dual-crosslinked Alg/OAlg/SF hydrogel increased from 28 to 67 kPa, due to the covalent imine bond formation. Then, MTT and DAPI staining assays were performed to demonstrate the biocompatibility of hydrogel. Furthermore, the differentiation potential of bone marrow mesenchymal stem cells encapsulated in hydrogel scaffolds to bone tissue was tested by ALP activity, Alizarin Red staining, and real-time PCR. The overall results showed the potential of Alginate/Oxidized Alginate/Silk Fibroin hydrogel scaffold for bone tissue engineering applications.
本研究旨在改善基于海藻酸盐的水凝胶的机械和生物学性能。为此，通过同时使用 CaCO-GDL 进行离子凝胶化和席夫碱反应，对 Alginate（Alg）/Oxidized Alginate（OAlg）/Silk Fibroin（SF）进行双重交联，制备了原位形成的水凝胶。通过 FTIR、SEM、压缩模量和流变学测试对所得水凝胶进行了表征。与物理交联的海藻酸盐水凝胶相比，由于形成了共价亚胺键，双重交联的 Alg/OAlg/SF 水凝胶的压缩模量从 28kPa 增加到 67kPa。然后，通过 MTT 和 DAPI 染色试验证明了水凝胶的生物相容性。此外，通过碱性磷酸酶（ALP）活性、茜素红染色和实时 PCR 测试，研究了包封在水凝胶支架中的骨髓间充质干细胞向骨组织分化的潜力。总体结果表明，Alginate/Oxidized Alginate/Silk Fibroin 水凝胶支架在骨组织工程应用中具有潜力。
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