Department of Biomedical Engineering, College of Engineering and Applied Sciences, Nanjing National Laboratory of Microstructures, Jiangsu Key Laboratory of Artificial Functional Materials, Nanjing University, Nanjing, Jiangsu 210023, China.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China.
Anal Chem. 2022 Aug 2;94(30):10636-10642. doi: 10.1021/acs.analchem.2c00804. Epub 2022 Jun 27.
Developing a universal strategy to measure catalase (CAT)/CAT-like activity, on one hand, overcomes limitations on current assays, such as moderate sensitivity and limited sample scope; on the other hand, facilitates insightful studies on applications of CAT and CAT-like nanozymes. Herein, the oxygen-sensitive and HO-inhibitory self-polymerization of dopamine (DA) was demonstrated as an activity indicator of CAT or CAT-like nanozymes, which monitors the catalytically generated O in a hypoxic environment. A typical assay for natural CAT was achieved under the optimized conditions. Moreover, this assay was suitable for diverse types of samples, ranging from nanozymes, animal tissues, to human saliva. By comparing the merits and limitations of common methods, this assay shows all-round advantages in sensitivity, specificity, and versatility, facilitating the formulation of measurement criteria and the development of potential standardized assays for CAT (or CAT-like nanozyme) activity.
开发一种通用策略来测量过氧化氢酶 (CAT)/CAT 样活性，一方面克服了当前测定方法的局限性，例如中等灵敏度和有限的样本范围；另一方面，有助于深入研究 CAT 和 CAT 样纳米酶的应用。本文中，多巴胺 (DA) 的氧敏感性和 HO 抑制自聚合被证明是 CAT 或 CAT 样纳米酶的活性指示剂，它可以监测缺氧环境中催化生成的 O。在优化条件下实现了对天然 CAT 的典型测定。此外，该测定法适用于多种类型的样本，包括纳米酶、动物组织和人唾液。通过比较常见方法的优缺点，该测定法在灵敏度、特异性和通用性方面具有全面优势，有利于制定测量标准，并开发潜在的 CAT（或 CAT 样纳米酶）活性标准化测定方法。
