Zeng Xiangle, Wang Hairong, Ma Yating, Xu Xue, Lu Xingxi, Hu Yujie, Xie Jihong, Wang Xiu, Wang Yushuai, Guo Xuliang, Zhao Li, Li Jianchun
School of Pharmacy, Bengbu Medical College, Bengbu, Anhui 233000, China.
Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Green Preparation and Application of Functional Materials, Hubei Key Laboratory of Polymer Materials, Hubei University, Wuhan, Hubei 430000, China.
ACS Appl Mater Interfaces. 2023 Mar 10. doi: 10.1021/acsami.2c20878.
Using tumors containing high concentrations of hydrogen peroxide to design nanozymes is a new and effective strategy, and vanadium-based nanomaterials receive increasing attention. In this paper, four kinds of vanadium oxide nanozymes with different valences of vanadium are synthesized by a simple method to verify the effect of valence on enzyme activity. Vanadium oxide nanozyme-III (Vnps-III) with a low valence of vanadium (V) exhibits good peroxidase (POD) and oxidase (OXD) activities, which can effectively produce reactive oxygen species (ROS) in the tumor microenvironment for tumor treatment. In addition, Vnps-III can also consume glutathione (GSH) to reduce ROS consumption. Vanadium oxide nanozyme-I (Vnps-I) containing a high valence of vanadium (V) has catalase (CAT) activity, which can catalyze hydrogen peroxide (HO) into oxygen (O), which is beneficial to alleviate the hypoxic environment of solid tumors. Finally, a vanadium oxide nanozyme with both trienzyme simulation activity and GSH consumption ability was screened out by adjusting the ratio of V to V in vanadium oxide nanozymes. In cell and animal experiments, we successfully demonstrate that vanadium oxide nanozymes have excellent antitumor ability and high safety, which may bring great potential for clinical cancer treatment.
利用含有高浓度过氧化氢的肿瘤来设计纳米酶是一种新颖且有效的策略，基于钒的纳米材料受到越来越多的关注。本文通过一种简单的方法合成了四种具有不同钒价态的氧化钒纳米酶，以验证价态对酶活性的影响。具有低价态钒（V）的氧化钒纳米酶III（Vnps-III）表现出良好的过氧化物酶（POD）和氧化酶（OXD）活性，能够在肿瘤微环境中有效产生活性氧（ROS）用于肿瘤治疗。此外，Vnps-III还能消耗谷胱甘肽（GSH）以减少ROS的消耗。含有高价态钒（V）的氧化钒纳米酶I（Vnps-I）具有过氧化氢酶（CAT）活性，可将过氧化氢（HO）催化分解为氧气（O），这有利于缓解实体瘤的缺氧环境。最后，通过调整氧化钒纳米酶中V与V的比例，筛选出了一种具有三酶模拟活性和GSH消耗能力的氧化钒纳米酶。在细胞和动物实验中，我们成功证明了氧化钒纳米酶具有优异的抗肿瘤能力和高安全性，这可能为临床癌症治疗带来巨大潜力。
