Sun Xiaoyan, Jiang Jing, Wang Gaofu, Zhou Peng, Li Jie, Chen Cancan, Liu Liangjia, Li Nianfu, Xia Yuanyou, Ren Hangxing
Chongqing Academy of Animal Sciences, Chongqing, 402460, China.
Chongqing Engineering Research Center for Goats, Chongqing, 402460, China.
Anim Biosci. 2023 Feb;36(2):191-199. doi: 10.5713/ab.21.0577. Epub 2022 Jun 24.
This study aimed to investigate the significant single nucleotide polymorphisms (SNPs) and genes associated with nine reproduction and morphological traits in three breed populations of Chinese goats.
The genome-wide association of nine reproduction and morphological traits (litter size, nipple number, wattle, skin color, coat color, black dorsal line, beard, beard length, and hind leg hair) were analyzed in three Chinese native goat breeds (n = 336) using an Illumina Goat SNP50 Beadchip.
A total of 17 genome-wide or chromosome-wide significant SNPs associated with one reproduction trait (litter size) and six morphological traits (wattle, coat color, black dorsal line, beard, beard length, and hind leg hair) were identified in three Chinese native goat breeds, and the candidate genes were annotated. The significant SNPs and corresponding putative candidate genes for each trait are as follows: two SNPs located on chromosomes 6 (CSN3) and 24 (TCF4) for litter size trait; two SNPs located on chromosome 9 (KATNA1) and 1 (UBASH3A) for wattle trait; three SNPs located on chromosome 26 (SORCS3), 24 (DYM), and 20 (PDE4D) for coat color trait; two SNPs located on chromosome 18 (TCF25) and 15 (CLMP) for black dorsal line trait; four SNPs located on chromosome 8, 2 (PAX3), 5 (PIK3C2G), and 28 (PLA2G12B and OIT3) for beard trait; one SNP located on chromosome 18 (KCNG4) for beard length trait; three SNPs located on chromosome 17 (GLRB and GRIA2), 28 (PGBD5), and 4 for hind leg hair trait. In contrast, there were no SNPs identified for nipple number and skin color.
The significant SNPs or genes identified in this study provided novel insights into the genetic mechanism underlying important reproduction and morphological traits of three local goat breeds in Southern China as well as further potential applications for breeding goats.
本研究旨在探究与中国山羊三个品种的九个繁殖和形态性状相关的显著单核苷酸多态性（SNP）和基因。
使用Illumina Goat SNP50 Beadchip对三个中国本地山羊品种（n = 336）的九个繁殖和形态性状（产仔数、乳头数、肉垂、肤色、毛色、背线、胡须、胡须长度和后腿毛）进行全基因组关联分析。
在三个中国本地山羊品种中，共鉴定出17个全基因组或染色体范围显著的SNP，它们与一个繁殖性状（产仔数）和六个形态性状（肉垂、毛色、背线、胡须、胡须长度和后腿毛）相关，并对候选基因进行了注释。每个性状的显著SNP和相应的假定候选基因如下：与产仔数性状相关的两个SNP分别位于6号染色体（CSN3）和24号染色体（TCF4）上；与肉垂性状相关的两个SNP分别位于9号染色体（KATNA1）和1号染色体（UBASH3A）上；与毛色性状相关的三个SNP分别位于26号染色体（SORCS3）、24号染色体（DYM）和20号染色体（PDE4D）上；与背线性状相关的两个SNP分别位于18号染色体（TCF25）和15号染色体（CLMP）上；与胡须性状相关的四个SNP分别位于8号染色体、2号染色体（PAX3）、5号染色体（PIK3C2G）和28号染色体（PLA2G12B和OIT3）上；与胡须长度性状相关的一个SNP位于18号染色体（KCNG4）上；与后腿毛性状相关的三个SNP分别位于17号染色体（GLRB和GRIA2）、28号染色体（PGBD5）和4号染色体上。相比之下，未鉴定出与乳头数和肤色相关的SNP。
本研究中鉴定出的显著SNP或基因，为中国南方三个本地山羊品种重要繁殖和形态性状的遗传机制提供了新见解，也为山羊育种提供了进一步的潜在应用。
