School of Food Science and Engineering, South China University of Technology, Guangzhou, Guangdong 510640, People's Republic of China; Research Institute for Food Nutrition and Human Health (111 Center), Guangzhou, Guangdong 510640, People's Republic of China.
School of Food Science and Engineering, South China University of Technology, Guangzhou, Guangdong 510640, People's Republic of China; Research Institute for Food Nutrition and Human Health (111 Center), Guangzhou, Guangdong 510640, People's Republic of China.
Food Res Int. 2022 Jul;157:111267. doi: 10.1016/j.foodres.2022.111267. Epub 2022 Apr 20.
In this study, a purified algal polysaccharide (P1) was isolated from Sargassum fusiforme and its structural characteristics and anti-photoaging activity were studied. Results showed that P1 had a molecular weight of 289 kDa and was mainly composed of mannuronic acid, guluronic acid and fucose with molar ratio of 7.67:2.35:1.00. The backbone of P1 was →4)-β-ManA-(1→4)-α-GulA-(1→4)-β-ManA-(1→4)-β-ManA-(1→4)-α-GulA-(1→4)-β-ManA-(1→3,4)-β-ManA-(1→ with a terminal group of α-Fucp-(1→ linked to O-3 position of →3,4)-β-ManA-(1→. In addition, P1 could inhibit the expressions of MMPs (MMP-1, MMP-3 and MMP-9) in the UVB-irradiated HaCaT cells, indicating that P1 could reduce collagen loss caused by UVB irradiation. It also reduced the contents of ROS and inflammatory factors (TNF-α, IL-6 and IL-1β), indicating that P1 could reduce the oxidative stress and inflammation response. Thus, Sargassum fusiforme polysaccharide P1 could be used as a potential functional food to relieve skin photoaging.
在这项研究中，从马尾藻中分离出一种纯化的海藻多糖（P1），并研究了其结构特征和抗光老化活性。结果表明，P1 的分子量为 289 kDa，主要由甘露糖醛酸、古洛糖醛酸和岩藻糖组成，摩尔比为 7.67:2.35:1.00。P1 的主链为→4)-β-ManA-(1→4)-α-GulA-(1→4)-β-ManA-(1→4)-β-ManA-(1→4)-α-GulA-(1→4)-β-ManA-(1→3,4)-β-ManA-(1→，末端基团为α-Fucp-(1→与→3,4)-β-ManA-(1→的 O-3 位相连。此外，P1 可以抑制 UVB 照射 HaCaT 细胞中 MMPs（MMP-1、MMP-3 和 MMP-9）的表达，表明 P1 可以减少由 UVB 照射引起的胶原蛋白损失。它还降低了 ROS 和炎症因子（TNF-α、IL-6 和 IL-1β）的含量，表明 P1 可以减轻氧化应激和炎症反应。因此，马尾藻多糖 P1 可用作缓解皮肤光老化的潜在功能性食品。
