Enhanced In Vitro Anti-Photoaging Effect of Degraded Seaweed Polysaccharides by UV/HO Treatment.

The high molecular weight and poor solubility of seaweed polysaccharides have limited their function and application. In this study, ultraviolet/hydrogen peroxide (UV/HO) treatment was used to prepare low-molecular-weight seaweed polysaccharides from . The effects of UV/HO treatment on the physicochemical properties and anti-photoaging activity of polysaccharides were studied. UV/HO treatment effectively degraded polysaccharides from (DSFPs), reducing their molecular weight from 271 kDa to 26 kDa after 2 h treatment. The treatment did not affect the functional groups in DSFPs but changed their molar percentage of monosaccharide composition and morphology. The effects of the treatment on the anti-photoaging function of polysaccharides were investigated using human epidermal HaCaT cells . DFSPs significantly improved the cell viability and hydroxyproline secretion of UVB-irradiated HaCaT cells. In particular, DSFP-45 obtained from UV/HO treatment for 45 min showed the best anti-photoaging effect. Moreover, DSFP-45 significantly increased the content and expression of collagen I and decreased those of pro-inflammatory cytokines, including interleukin-1β, interleukin-6, and tumor necrosis factor-α. Thus, UV/HO treatment could effectively improve the anti-photoaging activity of polysaccharides. These results provide some insights for developing novel and efficient anti-photoaging drugs or functional foods from seaweed polysaccharides.