Laboratory of Molecular Pharmacology, Department of Pharmacology, School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, China; Cell Therapy & Cell Drugs of Luzhou Key Laboratory, Luzhou, Sichuan, China.
Laboratory of Molecular Pharmacology, Department of Pharmacology, School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, China; Cell Therapy & Cell Drugs of Luzhou Key Laboratory, Luzhou, Sichuan, China; South Sichuan Institute of Translational Medicine, Luzhou, Sichuan, China.
Food Res Int. 2022 Jul;157:111401. doi: 10.1016/j.foodres.2022.111401. Epub 2022 May 26.
The dried roots of Pueraria lobata (Willd.) Ohwi as an edible medicinal herb are enriched with starch. However, the structure, physiology, and biological bioactivity of P. lobata starch (PLS) has not yet been fully investigated. This study showed that PLS consisted of mixed population of granules with polyhedral or spherical surface. The apparent content of resistant starch was 23.14%, and the molecular weight was 1.93 × 10 Da. PLS showed a branching degree and an average polymerization rate of 2.06% and 20.74%, respectively, with fairly high proportion of B1 short chains. The solubility and swelling power of PLS were 38.51% and 28.10 g/g, respectively, showing high hot stability of the viscosity. In vitro fermentation of PLS resulted in specifically altered composition of gut microbiota and increased production of SCFAs, showing a potential prebiotic effect. Moreover, PLS remarkably alleviated inflammation, hepatic steatosis and dyslipidemia in mice with high-fat high-cholesterol diet induced non-alcoholic fatty liver disease (NAFLD). The protective effect of PLS was associated with amelioration of NAFLD-associated gut dysbiosis through specifically increasing the abundance of Lactobacillus, Bifidobacterium and Turicibacter, and decreasing Desulfovibrio. The results would support the use of PLS as a functional prebiotic for protecting against NAFLD.
野葛的干根（野葛）作为一种可食用的药用植物，富含淀粉。然而，野葛淀粉（PLS）的结构、生理学和生物生物活性尚未得到充分研究。本研究表明，PLS 由具有多面或球形表面的颗粒的混合群体组成。抗性淀粉的表观含量为 23.14%，分子量为 1.93×10 Da。PLS 的分支度和平均聚合度分别为 2.06%和 20.74%，具有相当高比例的 B1 短链。PLS 的溶解度和溶胀能力分别为 38.51%和 28.10 g/g，表现出较高的热稳定性。PLS 的体外发酵导致肠道微生物群组成发生特定改变，并增加了 SCFAs 的产生，显示出潜在的益生元作用。此外，PLS 可显著减轻高脂肪高胆固醇饮食诱导的非酒精性脂肪性肝病（NAFLD）小鼠的炎症、肝脂肪变性和血脂异常。PLS 的保护作用与改善与 NAFLD 相关的肠道菌群失调有关，具体表现为增加乳酸杆菌、双歧杆菌和瘤胃球菌的丰度，减少脱硫弧菌的丰度。这些结果将支持将 PLS 用作预防 NAFLD 的功能性益生元。
