Tong Ping, Huang Fu-Rong, Xu Jun, Wu Zi-Qi, Hu Xing, Ling Ming, Wang Die, Wu Bu-Fei, Yang Du-Jiao, Zhang Ai-Min
Hunan Provincial Key Laboratory of Emergency and Critical Care Metabolomics, Hunan Provincial People's Hospital, Changsha 410000, China.
Zhongguo Dang Dai Er Ke Za Zhi. 2022 Jun 15;24(6):675-680. doi: 10.7499/j.issn.1008-8830.2112020.
To study the metabolic mechanism of neonatal sepsis at different stages by analyzing the metabolic pathways involving the serum metabolites with significant differences in neonates with sepsis at different time points after admission.
A total of 20 neonates with sepsis who were hospitalized in the Department of Neonatology, Hunan Provincial People's Hospital, from January 1, 2019 to January 1, 2020 were enrolled as the sepsis group. Venous blood samples were collected on days 1, 4, and 7 after admission. Ten healthy neonates who underwent physical examination during the same period were enrolled as the control group. Ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry was used for the metabonomic analysis of serum samples to investigate the change in metabolomics in neonates with sepsis at different time points.
On day 1 after admission, the differentially expressed serum metabolites between the sepsis and control groups were mainly involved in the biosynthesis of terpenoid skeleton. For the sepsis group, the differentially expressed serum metabolites between days 1 and 4 after admission were mainly involved in pyruvate metabolism, and those between days 4 and 7 after admission were mainly involved in the metabolism of cysteine and methionine. The differentially expressed serum metabolites between days 1 and 7 after admission were mainly involved in ascorbic acid metabolism.
The metabolic mechanism of serum metabolites varies at different stages in neonates with sepsis and is mainly associated with terpenoid skeleton biosynthesis, pyruvate metabolism, cysteine/methionine metabolism, and ascorbic acid metabolism.
通过分析脓毒症新生儿入院后不同时间点血清代谢物存在显著差异的代谢途径，研究新生儿脓毒症不同阶段的代谢机制。
选取2019年1月1日至2020年1月1日在湖南省人民医院新生儿科住院的20例脓毒症新生儿作为脓毒症组。入院后第1天、第4天和第7天采集静脉血样本。选取同期进行体检的10例健康新生儿作为对照组。采用超高效液相色谱-四极杆飞行时间质谱法对血清样本进行代谢组学分析，以研究脓毒症新生儿不同时间点的代谢组学变化。
入院后第1天，脓毒症组与对照组之间差异表达的血清代谢物主要参与萜类骨架的生物合成。对于脓毒症组，入院后第1天与第4天之间差异表达的血清代谢物主要参与丙酮酸代谢，入院后第4天与第7天之间差异表达的血清代谢物主要参与半胱氨酸和蛋氨酸代谢。入院后第1天与第7天之间差异表达的血清代谢物主要参与抗坏血酸代谢。
脓毒症新生儿血清代谢物的代谢机制在不同阶段有所不同，主要与萜类骨架生物合成、丙酮酸代谢、半胱氨酸/蛋氨酸代谢和抗坏血酸代谢有关。
