Khan Isna S, Faiyaz Zainab, Khan Asad U
Medical Microbiology and Molecular Biology Lab. Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh 202002, India.
Curr Protein Pept Sci. 2022;23(5):299-309. doi: 10.2174/1389203723666220627152112.
One of the greatest threats to the global world is infectious diseases. The morbidity and fatality of infectious diseases cause 17 million deaths annually. The recent COVID-19 pandemic describes the uncertain potential of these diseases. Understanding the pathogenesis of infectious agents, including bacteria, viruses, fungi, etc. and the evolution of rapid diagnostic techniques and treatments has become a pressing priority to improve infectious disease outcomes worldwide. Clustered regularly interspaced short palindromic repeats (CRISPR) constitute the adaptive immune system of archaea and bacteria along with CRISPR-associated (Cas) proteins that recognize and destroy foreign DNA acting as molecular scissors. Since their discovery, CRISPR systems are classified into 6 types and 22 subtypes. Type II, V, and VI are used for diagnostic purposes. Utilizing the CRISPR-Cas system's capabilities will aid promote the development of novel and improved diagnostics as well as innovative delivery systems and the prevention and treatment of infectious diseases.
传染病是全球面临的最大威胁之一。传染病的发病率和死亡率每年导致1700万人死亡。最近的新冠疫情凸显了这些疾病的潜在不确定性。了解包括细菌、病毒、真菌等在内的传染因子的发病机制以及快速诊断技术和治疗方法的发展，已成为改善全球传染病治疗效果的紧迫优先事项。成簇规律间隔短回文重复序列（CRISPR）与识别并破坏外来DNA的CRISPR相关（Cas）蛋白一起构成古细菌和细菌的适应性免疫系统，这些蛋白就像分子剪刀一样。自发现以来，CRISPR系统被分为6种类型和22个亚型。II型、V型和VI型用于诊断目的。利用CRISPR-Cas系统的能力将有助于促进新型和改进诊断方法以及创新递送系统的开发，以及传染病的预防和治疗。
