Division of Biotechnology, Entomology Research Institute, Loyola College, University of Madras, Chennai, India.
Xavier Research Foundation, St.Xavier's College, Palayamkottai, Tirunelveli, India.
Expert Rev Mol Diagn. 2021 Nov;21(11):1179-1189. doi: 10.1080/14737159.2021.1970535. Epub 2021 Nov 9.
Emerging novel infectious diseases and persistent pandemics with potential to destabilize normal life remain a public health concern for the whole world. The recent outbreak of pneumonia caused by Coronavirus infectious disease-2019 (COVID-19) resulted in high mortality due to a lack of effective drugs or vaccines. With a constantly increasing number of infections with mutated strains and deaths across the globe, rapid, affordable and specific detections with more accurate diagnosis and improved health treatments are needed to combat the spread of this novel pathogen COVID-19.
Researchers have started to utilize the recently invented clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (CRISPR/Cas)-based tools for the rapid detection of novel COVID-19. In this review, we summarize the potential of CRISPR/Cas system for the diagnosis and enablement of efficient control of COVID-19.
Multiple groups have demonstrated the potential of utilizing CRISPR-based diagnosis tools for the detection of SARS-CoV-2. In coming months, we expect more novel and rapid CRISPR-based kits for mass detection of COVID-19-infected persons within a fraction of a second. Therefore, we believe science will conquer COVID-19 in the near future.
新出现的传染病和可能破坏正常生活的持续大流行仍然是全世界公共卫生关注的焦点。最近由新型冠状病毒 2019 引起的肺炎爆发，由于缺乏有效的药物或疫苗，导致死亡率很高。由于全球感染变异株的数量不断增加和死亡人数不断增加，需要快速、经济实惠且具有特异性的检测方法，以更准确的诊断和改善的治疗方法来对抗这种新型病原体 COVID-19 的传播。
研究人员已经开始利用最近发明的成簇规律间隔短回文重复（CRISPR）和 CRISPR 相关蛋白（CRISPR/Cas）为基础的工具，快速检测新型 COVID-19。在这篇综述中，我们总结了 CRISPR/Cas 系统在 COVID-19 诊断和实现有效控制方面的潜力。
多个研究小组已经证明了利用基于 CRISPR 的诊断工具检测 SARS-CoV-2 的潜力。在未来几个月，我们预计会有更多新的和快速的基于 CRISPR 的试剂盒，可在几分之一秒内对 COVID-19 感染者进行大规模检测。因此，我们相信科学将在不久的将来战胜 COVID-19。
