School of Bioengineering, Dalian University of Technology, Dalian, Liaoning 116024, China.
School of Bioengineering, Dalian University of Technology, Dalian, Liaoning 116024, China.
Food Chem. 2022 Nov 15;394:133561. doi: 10.1016/j.foodchem.2022.133561. Epub 2022 Jun 23.
Combination of dietary flavonoid-baicalein and acarbose reduces the risk that prediabetes will develop into type 2 diabetes mellitus; however, the mechanism underlying this effect has not been clarified. In this study, the in vitro culture conditions of intestinal microorganisms from prediabetic mice were optimized to increase over 30% similarity between in vitro cultured and fecal samples. Baicalein and acarbose alone and in combination, and their corresponding starch hydrolysate were assayed by the in vitro model. The results indicated that the combination of baicalein with acarbose decreased gas production by reducing the residual starch ratio in starch hydrolysate and decreasing the dosage of acarbose, and that reducing the relative abundance of gut bacteria correlated with gas production is the main mechanism. This study provided a theoretical foundation for the development of flavonoid dietary supplements to enhance the efficacy of oral hypoglycemic agents with fewer side effects and higher efficacy.
饮食类黄酮-黄芩素与阿卡波糖的联合使用可降低糖尿病前期发展为 2 型糖尿病的风险；然而，其作用机制尚未阐明。在这项研究中，优化了来自糖尿病前期小鼠的肠道微生物的体外培养条件，使体外培养和粪便样本之间的相似度增加了 30%以上。通过体外模型检测黄芩素、阿卡波糖单独及联合使用及其相应的淀粉水解物。结果表明，黄芩素与阿卡波糖联合使用可通过降低淀粉水解物中残留淀粉的比例和减少阿卡波糖的剂量来减少气体产生，降低与气体产生相关的肠道细菌的相对丰度是主要机制。本研究为开发具有较少副作用和更高疗效的黄酮类膳食补充剂以增强口服降糖药的疗效提供了理论基础。
