Karami Ali Akbar, Javadi Amir, Salehi Sohrab, Nasirian Neda, Maali Amirhosein, Bakhshalizadeh Shadkam Maryam, Najari Masoumeh, Rousta Zahra, Alizadeh Safar Ali
Department of Urology, Qazvin University of Medical Sciences, Qazvin, Iran.
Department of Social Sciences, School of Medicine, Qazvin University of Medical Sciences Qazvin, Iran.
Iran J Microbiol. 2022 Apr;14(2):161-167. doi: 10.18502/ijm.v14i2.9182.
Prostatitis affects about 16% of men in their lifetime and sometimes leading to prostate cancer. Bacterial infections are the most common causes of prostatitis. Diagnosis of the causative agents of bacterial prostate infections plays an essential role in timely treating and preventing secondary complications. This study isolated bacterial infectious agents in patients' surgical prostate and evaluated them by routine and molecular microbiological methods.
In this cross-sectional study, 72 prostate biopsy specimens were collected from the Orology Departmen of hospitals of Qazvin University of Medical Sciences. All samples were cultured in aerobic and anaerobic conditions. Antibiotic susceptibility test by Kirby-Bauer standard method was performed for all isolated bacteria. In addition, all isolated bacteria were identified using 16S rDNA PCR and sanger sequencing methods. Also, TaqMan real-time PCR was applied to detect and
In conventional culture method, out of 18 positive samples, 15 samples (83.3%) were Gram-negative bacteria and 3 samples (16.6%) were Gram-positive bacteria, containing (55.5%), (11.1%), (5.5%), (11.1%), (11.1%), and (5.5%). The results of molecular identification methods were the same as conventional culture results. Also, four patients were , and three patients were positive for .
Most bacteria isolated from prostate specimens belonged to the family, especially and were cocci isolated in the specimens too. Also, and were identified in prostatitis.
前列腺炎在男性一生中的发病率约为16%，有时会导致前列腺癌。细菌感染是前列腺炎最常见的病因。诊断细菌性前列腺感染的病原体对于及时治疗和预防继发性并发症起着至关重要的作用。本研究从患者手术切除的前列腺中分离出细菌感染病原体，并通过常规和分子微生物学方法对其进行评估。
在这项横断面研究中，从加兹温医科大学医院泌尿外科收集了72份前列腺活检标本。所有样本均在需氧和厌氧条件下培养。采用 Kirby-Bauer 标准方法对所有分离出的细菌进行药敏试验。此外，使用16S rDNA PCR和桑格测序方法对所有分离出的细菌进行鉴定。同时，应用TaqMan实时PCR进行检测。
在传统培养方法中，18份阳性样本中，15份样本（83.3%）为革兰氏阴性菌，3份样本（16.6%）为革兰氏阳性菌，其中包含（55.5%）、（11.1%）、（5.5%）、（11.1%）、（11.1%）和（5.5%）。分子鉴定方法的结果与传统培养结果相同。此外，4例患者为，3例患者为阳性。
从前列腺标本中分离出的大多数细菌属于科，尤其是和也是标本中分离出的球菌。此外，和在前列腺炎中也被鉴定出来。
