Mirbagheri Firoozabad Maryam Sadat, Mohammadi Nasr Mina
Department of Biology, Faculty of Sciences, Yazd University, Yazd, Iran.
Department of Cell and Molecular Biology & Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran.
Iran J Microbiol. 2022 Apr;14(2):214-218. doi: 10.18502/ijm.v14i2.9190.
Human health and development have been related to dietary intake of essential fatty acids (omega 3, 6 and 9) and important for brain development, immune system function, and blood pressure regulation. Microbial essential oils are more natural and safer alternatives to synthetic preservatives. These oils have been demonstrated to have antibacterial activity within food systems and may be ideal additives to food formulations. Zygomycete fungi are well-known as good candidate for production of essential oils.
Essential oils of fungi and were extracted and fatty acids were analyzed by GC, for the first, antimicrobial activity of the fungi essential oils against foodborne pathogenic bacteria and was examined by disc diffusion and well diffusion methods and the minimal inhibitory concentrations (MIC) of oils were determined by microtiter plate.
The fungi oils were exhibited the stron g antibacterial effect against Gram-positive bacteria, and higher than Gram-negative and commercial oleic acid and linoleic acid. The MIC of the fungi oil extracts was 0.25 mg/ml for and and 0.5 mg/ml about . This research demonstrated microbial essential oils may be suitable for their antimicrobial properties in food.
Microbial essential oil with good antibacterial activity could also be used in selected cases like foodborne disease.
人类健康与发育与必需脂肪酸(欧米伽3、6和9)的饮食摄入有关,对大脑发育、免疫系统功能和血压调节很重要。微生物精油是合成防腐剂更天然、更安全的替代品。这些精油已被证明在食品体系中具有抗菌活性,可能是食品配方中的理想添加剂。接合菌纲真菌是众所周知的精油生产优良候选者。
首次提取了真菌和的精油,并通过气相色谱法分析脂肪酸,采用纸片扩散法和打孔扩散法检测真菌精油对食源性病原体和的抗菌活性,并用微量滴定板测定精油的最低抑菌浓度(MIC)。
真菌油对革兰氏阳性菌、表现出较强的抗菌作用,高于革兰氏阴性菌以及市售油酸和亚油酸。真菌油提取物对和的MIC为0.25毫克/毫升,对约为0.5毫克/毫升。本研究表明微生物精油因其在食品中的抗菌特性可能是合适的。
具有良好抗菌活性的微生物精油也可用于食源性疾病等特定情况。