一种在资源匮乏环境下采集和保存胎盘样本的新方法，用于鉴定和分析核酸。
A new method for the sampling and preservation of placental specimens in low-resource settings for the identification of and analysis of nucleic acids.
机构信息
Department of Anthropology, University of Michigan, Ann Arbor, MI, USA.
Research Center for Group Dynamics, Institute for Social Research, University of Michigan, Ann Arbor, MI, USA.
出版信息
J Histotechnol. 2022 Sep;45(3):116-119. doi: 10.1080/01478885.2022.2088191. Epub 2022 Jun 29.
Collection, preservation, and shipment of histological specimens in low-resource settings is challenging. We present a novel method that achieved excellent preservation of placental specimens from rural Mali by using formalin fixation, ethanol dehydration, and long-term storage in a solar-powered freezer. Sample preservation success was 92%, permitting evaluation of current and past malaria infection, anemia, placental maturity, and inflammation. Using RNAscope® hybridization we were able to visualize cell-specific gene expression patterns in the formalin-fixed paraffin-embedded (FFPE) specimens. Additionally, our method entailed mirrored sampling from the two cut faces of a cotyledon, one for the FFPE workflows and the other for storage in RNAlater™ and RNA-seq.
在资源匮乏的环境中收集、保存和运输组织学标本具有挑战性。我们提出了一种新方法，通过使用福尔马林固定、乙醇脱水以及太阳能冰箱长期储存，成功保存了马里农村地区的胎盘标本。样本保存成功率为 92%，可用于评估当前和过去的疟疾感染、贫血、胎盘成熟度和炎症情况。我们使用 RNAscope®杂交技术能够在福尔马林固定石蜡包埋（FFPE）标本中观察到细胞特异性基因表达模式。此外，我们的方法还包括从一个胎盘的两个切面进行镜像采样，一个用于 FFPE 工作流程，另一个用于储存在 RNAlater™和 RNA-seq 中。
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