Jiang Xiulin, Chen Xi, Guo Jishu, Zhou Fan, Pu Jun, Mutti Luciano, Niu Xiaoqun
Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Kunming College of Life Science, University of Chinese Academy of Sciences, Beijing, China.
Department of Neurosurgery, The Second Affiliated Hospital of Kunming Medical University, Kunming, China.
Front Mol Biosci. 2022 Jun 13;9:923584. doi: 10.3389/fmolb.2022.923584. eCollection 2022.
Lung adenocarcinoma (LUAD) is the most common histological lung cancer, and it is the leading cause of cancer-related deaths worldwide. Long non-coding RNAs (lncRNAs) have been implicated in the initiation and progression of various cancers. LncRNA-AC087588.2 (ENSG00000274976) is a novel lncRNA that is abnormally expressed in diverse cancer types, including LUAD. However, the clinical significance, prognostic value, diagnostic value, immune role, and the potential biological function of AC087588.2 LUAD remain elusive. In this study, we found that AC087588.2 was upregulated and associated with a poor prognosis in LUAD. In addition, univariate and multivariate Cox regression analysis indicated that AC087588.2 could be an independent prognostic factor for LUAD. Functionally, the knockdown of AC087588.2 restrained LUAD cell proliferation and migration . Finally, we constructed a ceRNA network that included hsa-miR-30a-5p and four mRNAs (ANLN, POLR3G, EHBP1, and ERO1A) specific to AC087588.2 in LUAD. The Kaplan-Meier survival analysis showed that lower expression of hsa-miR-30a-5p and higher expression of ANLN, POLR3G, EHBP1, and ERO1A were associated with adverse clinical outcomes in patients with LUAD. This finding provided a comprehensive view of the AC087588.2-mediated ceRNA network in LUAD, thereby highlighting its potential role in the diagnosis and prognosis of LUAD.
肺腺癌（LUAD）是最常见的组织学类型肺癌，也是全球癌症相关死亡的主要原因。长链非编码RNA（lncRNA）与多种癌症的发生和发展有关。LncRNA-AC087588.2（ENSG00000274976）是一种新型lncRNA，在包括LUAD在内的多种癌症类型中异常表达。然而，AC087588.2在LUAD中的临床意义、预后价值、诊断价值、免疫作用及潜在生物学功能仍不清楚。在本研究中，我们发现AC087588.2在LUAD中上调且与预后不良相关。此外，单因素和多因素Cox回归分析表明，AC087588.2可能是LUAD的独立预后因素。在功能上，敲低AC087588.2可抑制LUAD细胞增殖和迁移。最后，我们构建了一个ceRNA网络，该网络包含hsa-miR-30a-5p以及LUAD中AC087588.2特有的四个mRNA（ANLN、POLR3G、EHBP1和ERO1A）。Kaplan-Meier生存分析表明，hsa-miR-30a-5p低表达以及ANLN、POLR3G、EHBP1和ERO1A高表达与LUAD患者不良临床结局相关。这一发现提供了AC087588.2介导的LUAD中ceRNA网络的全面视图，从而突出了其在LUAD诊断和预后中的潜在作用。
