Molka Bouricha, Gwladys Bourdenet, Dorian Bosquet, Lucie Moussot, Mustapha Benkhalifa, Rosalie Cabry, Brigitte Gubler, Hafida Khorsi-Cauet, Moncef Benkhalifa
Reproductive Medicine, Reproductive Biology and Genetics, University Hospital and School of Medicine, Picardie University Jules Verne, Amiens, France.
Department of Immunology Laboratory, Amiens University Hospital, Amiens, France.
Front Physiol. 2022 Jun 13;13:859790. doi: 10.3389/fphys.2022.859790. eCollection 2022.
Growth hormone (GH) has gained attention as an anti-aging compound enhancing oocyte quality. In fact, GH is known to activate intrafollicular metabolic events for oocyte maturation. Insulin growth factor I (IGF1) is another ovarian growth factor that mediates the FSH and GH actions. Cytokines could also increase IVF outcomes. Indeed, IL-6 is a pleiotropic cytokine with multiple cellular effects that can vary based on the physiological environment. IL-6 may also play an important role in follicular development (Yang et al., J Assist Reprod Genet, 2020, 37 (5), 1171-1176). Clinical studies have been performed to explore the potential role of IL-6 in human oocyte maturation and subsequent embryonic development. To date, the answers are not conclusive. During peri-implantation, many cytokines balances are regulated like pro-inflammatory and anti-inflammatory interleukins. The pro-inflammatory properties of IL-17 and its impact on the tumor microenvironment or autoimmune diseases are characterized, but new dimensions of IL-17 activity that promotes embryo implantation are not well explored. In the search for answers, our study compared concentrations of growth factors IGF1, GH, and interleukins IL-6 and IL-17 in the follicular fluid (FF) from 140 women divided into two groups depending on bad (G1) or good prognosis (G2) and investigated the relationships between these FF components' levels and the main parameters of IVF. GH, IGF1, and IL-6 were significantly higher for G2. For GH, it was negatively correlated to patient age and positively correlated to maturity rate and IGF1. Moreover, GH and IGF1 were correlated to the top embryo rate and cumulative pregnancy rate. Regarding IL-6, it was correlated to IGF1 level, endometrium thickness, and implantation rate. As for IL-17, it was only correlated to IL-6. Consequently, all these FF components were predictive of oocyte quality except IL-17. GH seemed to be the best biomarker of this quality.
生长激素（GH）作为一种可提高卵母细胞质量的抗衰老化合物受到了关注。事实上，已知GH可激活卵泡内代谢事件以促进卵母细胞成熟。胰岛素生长因子I（IGF1）是另一种介导促卵泡激素（FSH）和GH作用的卵巢生长因子。细胞因子也可提高体外受精（IVF）成功率。实际上，白细胞介素-6（IL-6）是一种多效性细胞因子，具有多种细胞效应，其效应可能因生理环境而异。IL-6在卵泡发育中可能也起重要作用（Yang等人，《辅助生殖与遗传杂志》，2020年，37(5)，1171 - 1176）。已开展临床研究以探索IL-6在人类卵母细胞成熟及后续胚胎发育中的潜在作用。迄今为止，答案尚无定论。在植入前期，许多细胞因子平衡受到调节，如促炎和抗炎白细胞介素。白细胞介素-17（IL-17）的促炎特性及其对肿瘤微环境或自身免疫性疾病的影响已得到描述，但对促进胚胎植入的IL-17活性新方面的研究尚不充分。为寻找答案，我们的研究比较了140名女性卵泡液（FF）中生长因子IGF1、GH以及白细胞介素IL-6和IL-17的浓度，这些女性根据预后不良（G1）或预后良好（G2）分为两组，并研究了这些FF成分水平与IVF主要参数之间的关系。G2组的GH、IGF1和IL-6显著更高。对于GH，它与患者年龄呈负相关，与成熟率和IGF1呈正相关。此外，GH和IGF1与优质胚胎率和累积妊娠率相关。关于IL-6，它与IGF1水平、子宫内膜厚度和着床率相关。至于IL-17，它仅与IL-6相关。因此，除IL-17外，所有这些FF成分均可预测卵母细胞质量。GH似乎是这种质量的最佳生物标志物。
