Alzeeb George, Dubreuil Matthieu, Arzur Danielle, Rivet Sylvain, Corcos Laurent, Grand Yann Le, Le Jossic-Corcos Catherine
Univ Brest, Inserm, EFS, UMR 1078, GGB, F-29200 Brest, France.
Univ Brest, Laboratory of Optics and Magnetism OPTIMAG EA 938, F-29200 Brest, France.
Biomed Opt Express. 2022 Apr 28;13(5):3120-3130. doi: 10.1364/BOE.450518. eCollection 2022 May 1.
Gastric cancer (GC) is highly deadly. Three-dimensional (3D) cancer cell cultures, known as spheroids, better mimic tumor microenvironment (TME) than standard 2D cultures. Cancer-associated fibroblasts (CAF), a major cellular component of TME, promote or restrain cancer cell proliferation, invasion and resistance to drugs. We established spheroids from two human GC cell lines mixed with human primary CAF. Spheroid organization, analyzed by two-photon microscopy, showed CAF in AGS/CAF spheroids clustered in the center, but dispersed throughout in HGT-1/CAF spheroids. Such differences may reflect clonal specificities of GC cell lines and point to the fact that GC should be considered as a highly personalized disease.
胃癌（GC）极具致命性。三维（3D）癌细胞培养物，即球体，比标准的二维培养物能更好地模拟肿瘤微环境（TME）。癌症相关成纤维细胞（CAF）是TME的主要细胞成分，可促进或抑制癌细胞增殖、侵袭及耐药性。我们用人原发性CAF与两种人GC细胞系建立了球体。通过双光子显微镜分析球体结构，结果显示AGS/CAF球体中的CAF聚集在中心，而在HGT-1/CAF球体中则分散分布。这种差异可能反映了GC细胞系的克隆特异性，并表明GC应被视为一种高度个性化的疾病。
