Edelmann Marvin, Matamoros-Angles Andreu, Shafiq Mohsin, Pergament Mikhail, Glatzel Markus, Kärtner Franz X
Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607, Hamburg, Germany.
Department of Physics, Universität Hamburg, Jungiusstr. 9, 20355, Hamburg, Germany.
Sci Rep. 2025 Jul 8;15(1):24404. doi: 10.1038/s41598-025-08704-w.
Here, we present a fiber-optic dispersive wave generator for highly-efficient, wavelength-tunable ultrashort pulse generation, enabling multicolor deep-tissue two-photon imaging of neuronal and vascular structures in fixed, labeled mouse brain. Guided by comprehensive numerical simulations, a compact Yb: fiber laser-driven system is constructed that utilizes precisely parameter- and phase-matching-controlled dispersive wave generation in a photonic crystal fiber. The system delivers sub-100 fs pulses with over ~ 6.7 nJ of energy across a continuously tunable spectral range of 880-950 nm, achieving a record-high optical conversion efficiency of up to 65%. Optimizing the output for two-photon excitation of enhanced Green Fluorescent Protein and SYTOX Orange enables high-resolution structural imaging in mouse hippocampus and cerebellum at depths exceeding 450 μm. This technique for wavelength-tunable, high-energy and ultrashort pulse generation with record optical efficiency represents a significant advancement in ultrafast fiber laser technology for versatile biomedical two-photon imaging applications.
在此，我们展示了一种用于高效、波长可调谐超短脉冲产生的光纤色散波发生器，它能够对固定、标记的小鼠大脑中的神经元和血管结构进行多色深层组织双光子成像。在全面的数值模拟指导下，构建了一个紧凑的镱光纤激光驱动系统，该系统利用光子晶体光纤中精确的参数和相位匹配控制的色散波产生。该系统在880 - 950 nm的连续可调光谱范围内产生能量超过约6.7 nJ的亚100 fs脉冲，实现了高达65%的创纪录高光学转换效率。优化增强型绿色荧光蛋白和SYTOX Orange的双光子激发输出，能够在超过450μm深度的小鼠海马体和小脑中进行高分辨率结构成像。这种具有创纪录光学效率的波长可调谐、高能和超短脉冲产生技术代表了超快光纤激光技术在多功能生物医学双光子成像应用方面的重大进展。
