Zhang Yanyan, Zhang Ruiyu, Liu Penghong, Wang Jizhi, Gao Mingxue, Zhang Jie, Yang Jun, Yang Chunxia, Zhang Yu, Sun Ning
Department of Psychiatry, First Hospital of Shanxi Medical University, Taiyuan, China.
Department of Physiology, Shanxi Medical University, Taiyuan, China.
Front Neurosci. 2022 Jun 14;16:926450. doi: 10.3389/fnins.2022.926450. eCollection 2022.
Gut microbiota and childhood maltreatment are closely related to depressive symptoms. This study aimed to analyze the characteristics of gut microbiota in major depressive disorder (MDD) patients with childhood maltreatment experience and explore the correlation between gut microbiota, childhood maltreatment, and depressive symptoms. A total of 37 healthy controls (HCs) and 53 patients with MDD were enrolled, including 18 MDD patients without childhood maltreatment experience and 35 MDD patients with childhood maltreatment experience. The Hamilton's Depression Scale (HAMD-24) and Childhood Trauma Questionnaire-Short Form (CTQ-SF) were used to evaluate their depressive symptoms and childhood maltreatment experience, respectively. The composition of gut microbiota was evaluated using 16S rRNA sequencing. Spearman's correlation analysis was used to evaluate the correlation between different gut microbiota, depressive symptoms and childhood maltreatment. The mediation analysis was used to evaluate the mediating effect of gut microbiota. In the α-diversity analysis, we found that the Simpson index and Pielou's Evenness index differed significantly between MDD patients without childhood maltreatment experience and HCs. In the β-diversity analysis, principal coordinate analysis (PCoA) showed significant differences between MDD patients without childhood maltreatment experience, MDD patients with childhood maltreatment experience and HCs. Twenty-seven different bacteria were identified through Linear discriminant analysis effect size (LEfSe) analysis at different levels of classification. The analysis of the correlation showed that , and were significantly correlated with HAMD and CTQ-SF scores. The mediation analysis showed that childhood maltreatment had a significant direct effect on the patients' depressive symptoms, and had a significant mediating effect. The findings of this study suggested that MDD patients with childhood maltreatment experience had different gut microbiota, which might have a mediating effect on the influence of childhood maltreatment on depressive symptoms.
肠道微生物群与童年期虐待与抑郁症状密切相关。本研究旨在分析有童年期虐待经历的重度抑郁症（MDD）患者肠道微生物群的特征，并探讨肠道微生物群、童年期虐待与抑郁症状之间的相关性。共纳入37名健康对照者（HCs）和53名MDD患者，其中包括18名无童年期虐待经历的MDD患者和35名有童年期虐待经历的MDD患者。分别使用汉密尔顿抑郁量表（HAMD - 24）和儿童创伤问卷简表（CTQ - SF）评估他们的抑郁症状和童年期虐待经历。采用16S rRNA测序评估肠道微生物群的组成。使用Spearman相关性分析评估不同肠道微生物群、抑郁症状和童年期虐待之间的相关性。采用中介分析评估肠道微生物群的中介作用。在α多样性分析中，我们发现无童年期虐待经历的MDD患者与HCs之间的辛普森指数和皮洛均匀度指数存在显著差异。在β多样性分析中，主坐标分析（PCoA）显示无童年期虐待经历的MDD患者、有童年期虐待经历的MDD患者与HCs之间存在显著差异。通过线性判别分析效应大小（LEfSe）分析在不同分类水平上鉴定出27种不同的细菌。相关性分析表明， 与HAMD和CTQ - SF评分显著相关。中介分析表明，童年期虐待对患者的抑郁症状有显著的直接影响， 有显著的中介作用。本研究结果表明，有童年期虐待经历的MDD患者具有不同的肠道微生物群，这可能对童年期虐待对抑郁症状的影响具有中介作用。
