疏螺旋体的基因分析。

Genetic analysis of Borrelia.

作者信息

Hyde F W, Johnson R C

出版信息

Zentralbl Bakteriol Mikrobiol Hyg A. 1986 Dec;263(1-2):119-22. doi: 10.1016/s0176-6724(86)80111-0.

Abstract

DNA homology studies were performed on Borrelia hermsii, B. duttoni, B. crocidurae and the O. coriaceus spirochete. These organisms had a genus level relationship with each other. B. hermsii was 17 to 53% homologous with B. duttoni, B. crocidurae, and the O. coriaceus spirochetes; B. crocidurae was 17-32% related to B. hermsii, B. anserina and the O. coriaceus spirochete, and the O. coriaceus spirochete was 28-50% related to B. hermsii, B. crocidurae and B. anserina. The O. coriaceus spirochete was 10% homologous with B. burgdorferi, the etiologic agent of Lyme disease. Plasmid DNA was detected in these strains of Borrelia. Southern blot analysis of plasmid DNA from these strains suggested little or no homology with plasmid DNA from B. hermsii used as a probe. A plasmid of 9.2 kilobases was seen in B. burgdorferi strain CT1. This plasmid was shown to be a tandem repeat with unit length of 4.6 kilobases. Sequences corresponding to this plasmid were detected in several fresh tick isolates from North America, and were not seen in any animal, human or European isolates surveyed. The role in the biology of the spirochete played by this plasmid is unknown.

摘要

对赫氏疏螺旋体、达顿疏螺旋体、食虫疏螺旋体和科氏钝缘蜱螺旋体进行了DNA同源性研究。这些微生物在属水平上相互存在亲缘关系。赫氏疏螺旋体与达顿疏螺旋体、食虫疏螺旋体和科氏钝缘蜱螺旋体的同源性为17%至53%；食虫疏螺旋体与赫氏疏螺旋体、鹅疏螺旋体和科氏钝缘蜱螺旋体的相关性为17% - 32%，而科氏钝缘蜱螺旋体与赫氏疏螺旋体、食虫疏螺旋体和鹅疏螺旋体的相关性为28% - 50%。科氏钝缘蜱螺旋体与莱姆病的病原体伯氏疏螺旋体的同源性为10%。在这些疏螺旋体菌株中检测到了质粒DNA。对这些菌株的质粒DNA进行Southern印迹分析表明，与用作探针的赫氏疏螺旋体的质粒DNA几乎没有或没有同源性。在伯氏疏螺旋体CT1菌株中发现了一个9.2千碱基的质粒。该质粒显示为单位长度为4.6千碱基的串联重复序列。在来自北美的几个新鲜蜱虫分离株中检测到了与该质粒对应的序列，而在所调查的任何动物、人类或欧洲分离株中均未发现。该质粒在螺旋体生物学中的作用尚不清楚。