Habicht G S, Beck G, Benach J L, Coleman J L
Zentralbl Bakteriol Mikrobiol Hyg A. 1986 Dec;263(1-2):137-41. doi: 10.1016/s0176-6724(86)80115-8.
Lipopolysaccharides (LPS) are a constitutive part of the outer wall of gram negative bacteria. Because many of the symptoms of Lyme disease could be explained by a spirochetal LPS we have subjected Borrelia burgdorferi to standard LPS extraction techniques which yielded a LPS which accounted for 1.5-4% of the dry weight. The LPS was very similar to classical gram negative bacterial LPS both chemically and in its biological activities which included pyrogenicity, mitogenicity for lymphocytes and the induction of Interleukin 1 production by macrophages. In addition, the LPS produced an acute inflammatory reaction when injected intradermally into rabbit skin. It could also prepare a skin site for the production of the local Shwartzman reaction. These results show that the Lyme disease spirochete contains a hitherto unknown LPS that is biologically active in vitro and in vivo. It is likely that this molecule plays an important role in the pathogenesis of Lyme disease.
脂多糖（LPS）是革兰氏阴性菌外壁的组成部分。由于莱姆病的许多症状可以用螺旋体脂多糖来解释，我们对伯氏疏螺旋体采用了标准的脂多糖提取技术，得到了一种占干重1.5 - 4%的脂多糖。该脂多糖在化学和生物学活性方面与经典的革兰氏阴性菌脂多糖非常相似，其生物学活性包括致热性、对淋巴细胞的促有丝分裂性以及巨噬细胞诱导白细胞介素1的产生。此外，将该脂多糖皮内注射到兔皮肤中会引发急性炎症反应。它还能为局部施瓦茨曼反应的产生准备皮肤部位。这些结果表明，莱姆病螺旋体含有一种迄今未知的脂多糖，其在体外和体内均具有生物活性。这种分子很可能在莱姆病的发病机制中起重要作用。
