Sigal L H, Steere A C, Freeman D H, Dwyer J M
Arthritis Rheum. 1986 Jun;29(6):761-9. doi: 10.1002/art.1780290609.
In 27 patients with early Lyme disease, the mean response of peripheral blood mononuclear cells (PBMC) to Lyme spirochetal Borrelia burgdorferi antigens (723 counts per minute) was similar to that of control subjects. During convalescence, 2-3 weeks later, the patients' mean response was significantly higher (2,075 cpm, P less than 0.008). Compared with those with early disease, the PBMC of 22 patients with Lyme arthritis reacted even more to B burgdorferi (2,923 cpm, P less than 0.0004), and, by far, the greatest response was in concomitantly obtained synovial fluid mononuclear cells (15,238 cpm, P less than 0.001). The PBMC of patients with early Lyme disease reacted slightly less to phytohemagglutinin and pokeweed mitogen than those of normal control subjects, but patients with arthritis had greater than normal mitogen responses. In contrast, mitogen reactivity among synovial fluid cells was markedly decreased and correlated inversely with the response to antigen. Thus, in patients with Lyme disease, the antigen-specific responses of mononuclear cells increase as the disease progresses, and in those with arthritis, the greatest reactivity to antigen is found in cells in the inflamed joint.
在27例早期莱姆病患者中，外周血单个核细胞（PBMC）对莱姆螺旋体伯氏疏螺旋体抗原的平均反应（每分钟723计数）与对照受试者相似。在康复期，2至3周后，患者的平均反应显著升高（2075 cpm，P小于0.008）。与早期疾病患者相比，22例莱姆关节炎患者的PBMC对伯氏疏螺旋体的反应更强（2923 cpm，P小于0.0004），而迄今为止，最大反应出现在同时获取的滑液单个核细胞中（15238 cpm，P小于0.001）。早期莱姆病患者的PBMC对植物血凝素和商陆有丝分裂原的反应略低于正常对照受试者，但关节炎患者的有丝分裂原反应高于正常。相反，滑液细胞中的有丝分裂原反应性明显降低，且与对抗原的反应呈负相关。因此，在莱姆病患者中，单核细胞的抗原特异性反应随着疾病进展而增加，在关节炎患者中，对抗原的最大反应出现在炎症关节中的细胞中。
